Anti-Memo (N-terminal region) Antibody

Name: Anti-Memo (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: MP3721
Price: 275.0 USD
Availability: InStock

Our Memo (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects chicken, human, mouse, and rat Memo (N-terminal region) and is antigen affinity purified. It is great for use in WB, ICC.

Immunocytochemical labeling of Memo in rabbit spleen fibroblasts that were fixed in paraformaldehyde and permeabilized with NP-40. The cells were probed with the Memo (N-terminal Region) MP3721, then the antibody was detected using goat anti-rabbit DyLight® 594. The antibody was used in the absence (left) or presence (right) of it's blocking peptide (MX3725).

Western blot analysis of Memo expression in adult mouse heart (lane 1 & 4), mouse C2C12 cells (lane 2 & 5), and rabbit spleen fibroblast cells (lane 3 & 6). The blot was probed with anti-Memo (N-terminal region) (MP3721; lanes 1-6) in the presence (lanes 4-6) or absence (lanes 1-3) of Memo blocking peptide (MX3725).

Species Reactivity

Chicken, Human, Mouse, Rat

Applications

ICC, WB

Host Species

Rabbit

Go to Full Product Details

SKU: MP3721

Volume: 100 µL

Variant

Price:

$275.00

Quantity:

Product Details

Target

Memo (N-terminal region)

Target Description

During cell migration, actin assembly drives cell membrane protrusion, while microtubules (MTs) extend within protrusions to promote adhesion site turnover. Memo (mediator of ErbB2-driven cell motility) is an effector of the ErbB2 receptor tyrosine kinase involved in breast carcinoma cell migration. This effector may be important for mediating ErbB2-regulated changes in actin and MT dynamics during cell motility. Memo, a 297-amino-acid protein, has homology to class III nonheme iron-dependent dioxygenases, however it has not been shown to display metal binding or enzymatic activity. It has been shown to bind ErbB2 (Tyr-1227) phosphopeptide via its putative enzymatic active site. Memo and PLCγ1 interaction with ErbB2 is essential for HRG-induced chemotaxis. Furthermore, organization of the lamellipodial actin network is coordinated by signaling from Memo to the RhoA–mDia1 pathway localized to the plasma membrane. In addition, Memo may regulate actin dynamics by promoting cofilin depolymerizing and severing of F-actin

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Gene Name

MEMO1

Molecular Weight

Antigen

Memo synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human Memo. This peptide sequence is highly conserved in rat, mouse, and chicken Memo.

Species Reactivity

Chicken, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:200

Conjugate

Unconjugated

Specificity

The antibody detects a significant 33 kDa* protein corresponding to the molecular mass of Memo on SDS-PAGE immunoblots of mouse heart, mouse C2C12, and rabbit spleen fibroblasts.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

CGI27, c21orf19like, NS5ATP7

UniProt Details

UniProt (Human): Q9Y316
UniProt (Immunogen Species): Q9Y316

Recover password

Create my account

Anti-Memo (N-terminal region) Antibody

SKU: MP3721

Product Details

Bulk Order Anti-Memo (N-terminal region) Antibody

Related Products

Recently Viewed