Anti-mDia1 (N-terminal region) Antibody

Name: Anti-mDia1 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: DP4471
Price: 329.0 USD
Availability: InStock

2 Citations

Our mDia1 (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat mDia1 (N-terminal region) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

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SKU: DP4471

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Product Details

Target

mDia1 (N-terminal region)

Target Description

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia1 protein is activated by Rho and leads to ROCK-dependent stress fiber formation. Rho-activated mDia1 regulates serum response factor-dependent transcription. In cancers, mDia1 has been implicated in ras-mediated transformation, metastasis, and invasion. Thus, mDia1 is a Rho-activated formin with both cytoskeletal- and transcription-reguatling activities.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

DIAPH1

Molecular Weight

140

Antigen

mDia1 synthetic peptide corresponding to amino acids from the N-terminal region of human mDia1 (Diap1). This peptide sequence is highly conserved in rat and mouse mDia1, and has low homology to other formins.

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of mDia1 on SDS-PAGE immunoblots of human K562, human Jurkat, and mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

Dia1, Diaph1, DRF-1, Diap1, p140Dia, formin

UniProt Details

UniProt (Human): O60610
UniProt (Immunogen Species): O60610

Product Specific References for Applications and Species

Western Blot: Mouse

Western Blot: Mouse
PMID	Dilution	Publication
30294591	not listed	Thompson, S, et al. 2018. The Formin mDia1 Regulates Acute Lymphoblastic Leukemia Engraftment, Migration, and Progression in vivo. Frontiers in oncology, 389.
27707755	1:500	Ueyama, T, et al. 2016. Constitutive activation of DIA1 (DIAPH1) via C-terminal truncation causes human sensorineural hearing loss. EMBA Molecular Medicine, 1310-1324.

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Anti-mDia1 (N-terminal region) Antibody

SKU: DP4471

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-mDia1 (N-terminal region) Antibody

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