Anti-mDia1 (N-terminal region) Antibody

Name: Anti-mDia1 (N-terminal region) Antibody
Brand: PhosphoSolutions
SKU: DP4471
Price: 348.0 USD
Availability: InStock

Our mDia1 (N-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat mDia1 (N-terminal region) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of mDia1 expression in human Jurkat cells (lanes 1-4). The blots were probed with anti-mDia1 (a.a. 66-77: DP4471) in the presence (lane 1) or absence of blocking peptide (DX4475) using dilutions of 1:250 (lane 2), 1:1000 (lane 3), and 1:4000 (lane 4).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: DP4471

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

mDia1 (N-terminal region)

Scientific Background

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia1 protein is activated by Rho and leads to ROCK-dependent stress fiber formation. Rho-activated mDia1 regulates serum response factor-dependent transcription. In cancers, mDia1 has been implicated in ras-mediated transformation, metastasis, and invasion. Thus, mDia1 is a Rho-activated formin with both cytoskeletal- and transcription-reguatling activities.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

DIAPH1

Molecular Weight

140

Immunogen

mDia1 synthetic peptide corresponding to amino acids from the N-terminal region of human mDia1 (Diap1). This peptide sequence is highly conserved in rat and mouse mDia1, and has low homology to other formins.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 140 kDa* protein corresponding to the apparent molecular mass of mDia1 on SDS-PAGE immunoblots of human K562, human Jurkat, and mouse C2C12 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

DFNA1; Dia1; DRF1; LFHL1; SCBMS; hDIA1; protein diaphanous homolog 1; Diaph1; DRF-1; Diap1; p140Dia; formin

UniProt ID

O60610
UniProt Summary: Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration. Has neurite outgrowth promoting activity. Acts in a Rho-dependent manner to recruit PFY1 to the membrane. In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape. Plays a role in brain development. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.

NCBI Gene ID

1729

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Anti-mDia1 (N-terminal region) Antibody

SKU: DP4471

Product Details

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