Anti-mCherry Antibody
Our Anti-mCherry chicken polyclonal primary antibody detects mCherry, and is concentrated purified IgY. It is validated for use in ICC, WB.
![Left: HEK293 cells transfected with a mCherry (red) were stained with chicken antibody to mCherry (green). Blue: DAPI nuclear stain. Transfected cells appear bright red, and co-stain for mCherry chicken antibody. Superimposition of both signals results in yellow-orange colour. Right: Western blot analysis of transfected and control HEK293 cell lysates, and recombinant proteins with chicken antibody to mCherry (1:2,000, green). [1] protein standard, [2] HEK293 control, [3] HEK293 cells transfected with mCherry, [4] recombinant mCherry protein, [5] recombinant GFP protein, [6] HEK293 transfected with GFP. The major band at about 30 kDa corresponds to mCherry protein and the slightly larger recombinant form runs at about 33 kDa due to presence of a His tag and other vector derived sequences. No cross-reactivity with GFP protein is observed. The same blot was simultaneously probed with a mouse antibody to HSP60 (red), which reveals a band at 60 kDa only in cell lysates.](http://www.antibodiesinc.com/cdn/shop/files/C-1655-100-ihc-wb_120x120.jpg?v=1759274145)
A: Western blot analysis of HEK293 cells transfected with pFin-EF1-mCherry vector. C-1655-100 (1:2,000 dilution) reveals a strong protein band in transfected cells (Lane 1). Non-transfected control cells (Lane 2) show no mCherry protein band.
B: Immunofluorescence analysis of mCherry-transfected HEK293 cells with C-1655-100. Specificity of C-1655-100 for mCherry is shown by superimposing mCherry fluorescence (red) with C-1655-100 antibody staining (green), which results in a yellow color. mCherry staining is also seen in the nucleus, which might be due to degradation of mCherry molecules, causing the release of the low molecular weight mCherry fluorochrome.
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B: Immunofluorescence analysis of mCherry-transfected HEK293 cells with C-1655-100. Specificity of C-1655-100 for mCherry is shown by superimposing mCherry fluorescence (red) with C-1655-100 antibody staining (green), which results in a yellow color. mCherry staining is also seen in the nucleus, which might be due to degradation of mCherry molecules, causing the release of the low molecular weight mCherry fluorochrome.
