"Endogenous and transfected cell immunoblot: extracts of HEK-293 cells transfected with GFP-tagged LRRK2-WT, LRRK2-S910A or LRRK2-S935A plasmid; fibroblasts from embryonic WT or LRRK2 KO mice untreated or treated with LRRK2-IN-1 inhibitor (2011 Deng et al Nat Chem Biol); and human lymphoblasts untreated or treated with LRRK2-IN-1, probed with 1D8. Data courtesy of Nicolas Dzamko, Paul Davies and Dario Alessi (University of Dundee, Scotland, UK)."
Bulk Order Anti-LRRK2/Dardarin-pSer910 Antibody
LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.
Purified by Protein A chromatography
Synthetic peptide amino acids 904-917 (VKKKSN[pS]ISVGEFY) of human LRRK2 (accession number Q5S007).
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
No cross-reactivity reported
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.