Anti-LRP4 (Extracellular) Antibody (N207/27)

from NeuroMab Datasheet
19 Citations

Available Conjugates

490
550
594
650

Primary Antibody
Human, Mouse, Rabbit, Rat
N207/27
ICC, IHC, WB
Mouse
Purified by Protein A chromatography
Lrp4 Kiaa0816
215 kDa

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Adult rat brain membrane immunoblot
Representative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat no 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Western blot image of mouse skeletal muscle confirming of expression of Flag-LRP4 (catalog no 75-221, 1:1000). Image from publication CC-BY-4.0. PMID:36765071
Rat ColQ−/− muscle cells were transduced to express ColQ-Myc (+) or not (−). After differentiation, the myotubes were treated (+) or not (−) with 10 nM of neural agrin for 1 h, and MuSK tyrosine phosphorylation was assessed as aforementioned. E, MuSK and LRP4 (cat. 75-221, 1:1000) cs levels of ColQ−/− myotubes re-expressing ColQ-Myc (+) or not (−) and not treated with agrin were analyzed. Image from publication CC-BY-4.0. PMID:37356721
Adult rat brain membrane immunoblot
Adult rat brain membrane immunoblot
Representative images of EDL muscles of 3 M, 12 M, and 24 M-old mice, whole mount stained with 555-anti-LRP4 (Cat no 75-221, 1:200, red) and NF/Syn (blue). Arrowheads, non-annulospiral axons had no LRP4 expression. Arrows, LRP4 negative annulospiral endings in aged mice. Scale bar, 10 µm. Image from publication CC-BY-4.0. PMID:36765071
Western blot image of mouse skeletal muscle confirming of expression of Flag-LRP4 (catalog no 75-221, 1:1000). Image from publication CC-BY-4.0. PMID:36765071
Rat ColQ−/− muscle cells were transduced to express ColQ-Myc (+) or not (−). After differentiation, the myotubes were treated (+) or not (−) with 10 nM of neural agrin for 1 h, and MuSK tyrosine phosphorylation was assessed as aforementioned. E, MuSK and LRP4 (cat. 75-221, 1:1000) cs levels of ColQ−/− myotubes re-expressing ColQ-Myc (+) or not (−) and not treated with agrin were analyzed. Image from publication CC-BY-4.0. PMID:37356721

Anti-LRP4 (Extracellular) Antibody
SKU: 75-221

Volume: 100 µL
1-2 business days
Price:
$308

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Product Specific References for Applications and Species

Immunohistochemistry: Mouse
PMID Dilution Publication
367650711:200Cao, R., et al. 2023. Intrafusal-fiber LRP4 for muscle spindle formation and maintenance in adult and aged animals. Nature Communications, 744.
312684201:1000Zhang, H., et al. 2019. Agrin-Lrp4-Ror2 signaling regulates adult hippocampal neurogenesis in mice.. Elife, e45303.
Patch Clamp Recording: Mouse
PMID Dilution Publication
24462100not listedWang, K., et al. 2014. Distinct Ca2+ sources in dendritic spines of hippocampal CA1 neurons couple to SK and Kv4 channels.. Neuron, 379-387.
Solid Phase Binding Assay: Human
PMID Dilution Publication
34233932not listedYu, Z., et al. 2021. Characterization of LRP4/agrin antibodies from a patient with myasthenia gravis. Neurology, e975-e987.
Western Blot: Mouse
PMID Dilution Publication
367650711:1000Cao, R., et al. 2023. Intrafusal-fiber LRP4 for muscle spindle formation and maintenance in adult and aged animals. Nature Communications, 744.
340905161:500Jing, H., et al. 2021. Synapse-specific Lrp4 mRNA enrichment requires Lrp4/MuSK signaling, muscle activity and Wnt non-canonical pathway. Cell Bioscience, 105.
340639921:1000Hui, T.K., et al. 2021. Ablation of Lrp4 in Schwann cells promotes peripheral nerve regeneration in mice. Biology (Basel), 452.
339876571:1000Hui, T., et al. 2021. Increasing LRP4 diminishes neuromuscular deficits in a mouse model of Duchenne muscular dystrophy. Human Molecular Genetics, 1579-1590.
326876711:1000Chen, A., et al. 2020. APC2 CDH1 negatively regulates agrin signaling by promoting the ubiquitination and proteolytic degradation of DOK7. FASEB, 12009-12023.
324570761:1000Zhang, H., et al. 2020. A role of low-density lipoprotein receptor-related protein 4 (LRP4) in astrocytic Aβ clearance.. Journal of Neuroscience, 5347-5361.
312613761:1000Xiao, Y., et al. 2020. Loss of mitochondrial protein CHCHD10 in skeletal muscle causes neuromuscular junction impairment. Human Molecular Genetics, 1784-1796.
28193701not listedXiong, L., et al. 2017. Osteoblastic Lrp4 promotes osteoclastogenesis by regulating ATP release and adenosine-A2AR signaling.. The Journal of Biology, 761-778.
272945131:1000Sun, X.D., et al. 2016. Lrp4 in astrocytes modulates glutamatergic transmission. Nature Neuroscience, 1010-1018.
254076771:2500Gomez, A.M., et al. 2014. Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.. Elife, e04287.
253196861:1000Barik, A., et al. 2013. LRP4 is critical for neuromuscular junction maintenance.. Journal of Neuroscience, 13892-13905.
242006891:1000Shen, C., et al. 2013. Antibodies against low-density lipoprotein receptor–related protein 4 induce myasthenia gravis. Journal of Clinical Investigation, 5190-5202.
227942641:1000Wu, H., et al. 2012. Distinct roles of muscle and motoneuron LRP4 in neuromuscular junction formation.. Neuron, 94-107.
Western Blot: Rabbit
PMID Dilution Publication
242006891:1000Shen, C., et al. 2013. Antibodies against low-density lipoprotein receptor–related protein 4 induce myasthenia gravis. Journal of Clinical Investigation, 5190-5202.
Western Blot: Rat
PMID Dilution Publication
373567211:1000Uyen Dao, TM, et al. 2023. The Collagen ColQ Binds to LRP4 and Regulates the Activation of the Muscle-Specific Kinase/LRP4 Receptor Complex by Agrin at the Neuromuscular Junction. The Journal of Biological Chemistry, 104962.

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