Anti-Lamin A/C Antibody

Name: Anti-Lamin A/C Antibody
Brand: Biosensis
SKU: C-1698-100
Price: 337.0 USD
Availability: InStock

Our Anti-Lamin A/C chicken polyclonal primary antibody detects human Lamin A/C, and is affinity-purified. It is validated for use in ICC, IHC, WB.

Crude HeLa cell extract stained with Chicken anti-Lamin A/C. Two bands at 74 kDa and 65 kDa, corresponding to Lamin A and C.

HeLa cells stained with C-1698-100 (red), and counterstained with our monoclonal antibody to LAMP1 (M-1652-100). The Lamin A/C antibody reveals strong nuclear lamina staining, while the LAMP1 antibody reveals strong cytoplasmic punctate staining of lysosomes and early endosomes. The blue stain reveals DNA in the nuclei of these cells. Since both DNA (blue) and Lamin A/C (red) are associated with the nuclear compartment, this region appears crimson in this image.

$Left: Analysis of lamin A/C expression in HeLa cells with chicken anti-lamin A/C (red, 1:2,000), costained with mouse anti-actin (green) by Immunocytochemistry. Blue: Hoechst nuclear DNA stain. The chicken antibody specifically labels the nuclear lamina, while the actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites. Right: Western blot analysis of cytosolic or nuclear enriched fractions of cell lines probed with chicken anti-lamin A/C (green, 1:1,000). [2] HeLa cytosol, [3] HeLa nuclear, [4] NIH-3T3 cytosol, and [5] NIH-3T3 nuclear fractions. Two strong bands at 65 kDa and 74 kDa correspond to lamin A and lamin C proteins, respectively, detected exclusively in the nuclear fractions. The same blot was simultaneously probed with mouse anti-GAPDH (M-1376-250, red). The single band at 37 kDa represents GAPDH protein$

Crude HeLa cell extract stained with Chicken anti-Lamin A/C. Two bands at 74 kDa and 65 kDa, corresponding to Lamin A and C.

Click on image to zoom

Species Reactivity

Human

Applications

ICC, IHC, WB

Host Species

Chicken

Go to Full Product Details

Data Sheet

SKU: C-1698-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Lamin A/C

Scientific Background

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes 98 amino acids missing from Lamin C, while Lamin C has a C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other, as is the case with this monoclonal. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3).

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

74 kDa and 65 kDa

Immunogen

Full length recombinant human Lamin C

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:1000-1:2000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

Lamin A and Lamin C. The antibody reacts with a 74 kDa and 65 kDa band by Western blot on HeLa cell extract. It has also been used successfully for immunocytochemistry on HeLa cell cultures.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

70 kDa lamin; 70 kDa lamin; Cardiomyopathy dilated 1A (autosomal dominant; Renal carcinoma antigen NY-REN-32; CDCD1; LMNA; CDDC; LMN1; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; Lamin A; Lamin A/C; Lamin A/C like 1; Lamin; Lamin C; Lamin-A/C; LDP1; LFP; LGMD1B; Limb girdle muscular dystrophy 1B (autosomal dominant; LMN 1; LMN A; LMN C; LMN1; LMNA; LMNA_HUMAN; LMNC; LMNL1; Prelamin A/C; PRO1; Renal carcinoma antigen NY REN 32; Renal carcinoma antigen NY-REN-32; Renal carcinoma antigen NYREN32

UniProt ID

P02545
UniProt Summary: [Lamin-A/C]: Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. Lamin A and C are present in equal amounts in the lamina of mammals. Also involved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone. Required for cardiac homeostasis.

NCBI Gene ID

4000

Shipping

25°C (ambient)

Recover password

Create my account

Anti-Lamin A/C Antibody

SKU: C-1698-100

Product Details

Bulk Order Anti-Lamin A/C Antibody

Related Products

Recently Viewed