Anti-Kv7.4/KCNQ4 K+ Channel Antibody (N43/6)
Our Anti-Kv7.4/KCNQ4 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N43/6. It detects bovine, human, mouse, and rat Kv7.4/KCNQ4 K+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM (left lane) and COS-1 cells transiently transfected with KCNQ4 (middle lane) or Kv2.1 (right lane) plasmids and probed with pure N43/6 (left panel) or K89/41 TC supe (right panel).
Product Specific References for Applications and Species
- Immunocytochemistry: Human | Mouse | Rat
- Immunohistochemistry: Human | Rat
- Immunoprecipitation: Human | Mouse
- PLA: Rat
- Western Blot: Bovine | Human | Mouse | Rat
| Immunocytochemistry: Human | ||
| PMID | Dilution | Publication |
| 23324056 | 1:500 | David, J., et al. 2013. Trafficking of the IKs -complex in MDCK cells: site of subunit assembly and determinants of polarized localization. Traffic (Copenhagen, Denmark), 399-411. |
| 20966080 | not listed | Kim, H., et al. 2011. Cellular and molecular mechanisms of autosomal dominant form of progressive hearing loss, DFNA2. The Journal of biological chemistry, 1517-1527. |
| Immunocytochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 35085542 | 1:100 | Paventi, G., et al. 2022. Kv7.4 channels regulate potassium permeability in neuronal mitochondria. Biochemical pharmacology, 114931. |
| Immunocytochemistry: Rat | ||
| PMID | Dilution | Publication |
| 33364977 | 1:200 | Baldwin, S., et al. 2020. KV7 Channel Expression and Function Within Rat Mesenteric Endothelial Cells. Frontiers in physiology, 598779. |
| 26718475 | 1:100 | Testai, L., et al. 2016. Expression and function of Kv7.4 channels in rat cardiac mitochondria: possible targets for cardioprotection. Cardiovascular research, 40-50. |
| 26503181 | 1:200 | Jepps, T., et al. 2015. Fundamental role for the KCNE4 ancillary subunit in Kv7.4 regulation of arterial tone. The Journal of physiology, 5325-5340. |
| Immunohistochemistry: Human | ||
| PMID | Dilution | Publication |
| 24120659 | 1:50 | Adduci, A., et al. 2013. Expression and motor functional roles of voltage-dependent type 7 K(+) channels in the human taenia coli. European journal of pharmacology, 12-20. |
| 20040580 | 1:100 | Iannotti, F., et al. 2010. Expression, localization, and pharmacological role of Kv7 potassium channels in skeletal muscle proliferation, differentiation, and survival after myotoxic insults. The Journal of pharmacology and experimental therapeutics, 811-820. |
| Immunohistochemistry: Rat | ||
| PMID | Dilution | Publication |
| 33364977 | 1:200 | Baldwin, S., et al. 2020. KV7 Channel Expression and Function Within Rat Mesenteric Endothelial Cells. Frontiers in physiology, 598779. |
| 25965962 | not listed | Salomonsson, M., et al. 2015. K(V)7.4 channels participate in the control of rodent renal vascular resting tone. Acta physiologica (Oxford, England), 204-214. |
| 24109455 | 0.6μg/ml | Evseev, A., et al. 2013. Functional effects of KCNQ K(+) channels in airway smooth muscle. Frontiers in physiology, 277. |
| 26802314 | 1:200 | Jepps, T., et al. 2016. Molecular and functional characterization of Kv 7 channels in penile arteries and corpus cavernosum of healthy and metabolic syndrome rats. British journal of pharmacology, 1478-1490. |
| Immunoprecipitation: Human | ||
| PMID | Dilution | Publication |
| 26515070 | 1:500 | Sihn, C., et al. 2016. Mechanisms of Calmodulin Regulation of Different Isoforms of Kv7.4 K+ Channels. The Journal of biological chemistry, 2499-2509. |
| Immunoprecipitation: Mouse | ||
| PMID | Dilution | Publication |
| 24927567 | not listed | Iannotti, F., et al. 2014. The endocannabinoid 2-AG controls skeletal muscle cell differentiation via CB1 receptor-dependent inhibition of Kv7 channels. Proceedings of the National Academy of Sciences of the United States of America, E2472-2481. |
| PLA: Rat | ||
| PMID | Dilution | Publication |
| 31992990 | 1:200 | Greenwood, I., et al. 2020. The Gβ1 and Gβ3 Subunits Differentially Regulate Rat Vascular Kv7 Channels. Frontiers in physiology, 1573. |
| 30002060 | 1:200 | Stott, J., et al. 2018. Investigating the Role of G Protein βγ in Kv7-Dependent Relaxations of the Rat Vasculature. Arteriosclerosis, thrombosis, and vascular biology, 2091-2102. |
| 26503181 | 1:200 | Jepps, T., et al. 2015. Fundamental role for the KCNE4 ancillary subunit in Kv7.4 regulation of arterial tone. The Journal of physiology, 5325-5340. |
| Western Blot: Bovine | ||
| PMID | Dilution | Publication |
| 24416770 | 1:400 | Zhang, X., et al. 2013. KCNQ and KCNE potassium channel subunit expression in bovine retinal pigment epithelium. Experimental eye research, 424-432. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 27761601 | 1:1000 | Bientinesi, R., et al. 2017. KV7 channels in the human detrusor: channel modulator effects and gene and protein expression. Naunyn-Schmiedeberg's archives of pharmacology, 127-137. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 35085542 | 1:500 | Paventi, G., et al. 2022. Kv7.4 channels regulate potassium permeability in neuronal mitochondria. Biochemical pharmacology, 114931. |
| 23242999 | 1:500 | Iannotti, F., et al. 2013. Specification of skeletal muscle differentiation by repressor element-1 silencing transcription factor (REST)-regulated Kv7.4 potassium channels. Molecular biology of the cell, 274-284. |
| Western Blot: Rat | ||
| PMID | Dilution | Publication |
| 26718475 | 1:500-1:1000 | Testai, L., et al. 2016. Expression and function of Kv7.4 channels in rat cardiac mitochondria: possible targets for cardioprotection. Cardiovascular research, 40-50. |
| 25361569 | not listed | Sedivy, V., et al. 2015. Role of Kv7 channels in responses of the pulmonary circulation to hypoxia. American journal of physiology. Lung cellular and molecular physiology, L48-57. |
| 21895977 | 1:500 | Svalø, J., et al. 2012. Kv 7 positive modulators reduce detrusor overactivity and increase bladder capacity in rats. Basic & clinical pharmacology & toxicology, 145-153. |