Anti-Kv1.1 K+ Channel (External) Antibody (K36/15)

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Our Anti-Kv1.1 K+ channel (external) mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone K36/15. It is KO validated, detects mouse and rat Kv1.1 K+ channel (external), and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with K36/15 (left) or N52A/42 (right) TC supe.
Immunofluorescence staining of hippocampus from adult Kv1.1 wildtype (left) or knock-out (right) mouse with K36/15 (red) and K13/31 (green, Kv1.4).
Representative confocal micrographs of paranode (CASPR, brace; red) pairs with interspersed node of Ranvier (bracket), demarcated by juxtaparanodes (KV1.1; white) in brain from with standard-housed (SH) or environmental enrichment (EE) mice. Image from publication CC-BY-4.0. PMID:36217300
Adult brain membrane immunoblot.
Low magnification image of mouse cerebellar cortex folium section showing KV1.1 (cat. 75-105, 1:1000) immunofluorescence . Right. High magnification confocal image showing concentrated KV1.1 immunofluorescence (green) localized at the interface of Purkinje and granule cell layers. Right lower. Same image showing co-immunofluorescence labelling for the Purkinje cell marker calbindin (magenta). Image from publication CC-BY-4.0. PMID: 37408217
Immunoblot against crude brain membranes from adult rat (RBM) and Kv1.1 wild-type (WT) or knock-out (KO) mouse probed with K36/15 (left) or N52A/42 (right) TC supe.
Representative confocal sections of lamina adjacent to the Purkinje cell layer from Kcna1V408A/+ and control cerebellar cortex, labelled for KV1.1 (Cat.75-105, 1:1000; yellow) and PSD-95 (centre, magenta). Right, merged image. Image from publication CC-BY-4.0. PMID: 37408217
Immunolabeling of postnatal 14 mouse CA1 (Axonal Initial Segment, Interneuron) specifically labeling extracellular Kv1.1 (Cat no 75-105, 1:200, green) and Ankyrin G (red). DNA labeled with DAPI. Mice transcardially perfused with cold 4% PFA in PBS, brain removed and postfixed overnight. 50 uM slices as floating sections, antigen retrieval performed with sodium citrate at 80 C for 30 min before immunolabeling. Images from: J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.
Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with K36/15 (left) or N52A/42 (right) TC supe.
Roll over image to zoom in Click on image to zoom
Immunoblot versus crude brain membranes from adult mouse (MBM), rat (RBM) and human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)] probed with K36/15 (left) or N52A/42 (right) TC supe.
Immunofluorescence staining of hippocampus from adult Kv1.1 wildtype (left) or knock-out (right) mouse with K36/15 (red) and K13/31 (green, Kv1.4).
Representative confocal micrographs of paranode (CASPR, brace; red) pairs with interspersed node of Ranvier (bracket), demarcated by juxtaparanodes (KV1.1; white) in brain from with standard-housed (SH) or environmental enrichment (EE) mice. Image from publication CC-BY-4.0. PMID:36217300
Adult brain membrane immunoblot.
Low magnification image of mouse cerebellar cortex folium section showing KV1.1 (cat. 75-105, 1:1000) immunofluorescence . Right. High magnification confocal image showing concentrated KV1.1 immunofluorescence (green) localized at the interface of Purkinje and granule cell layers. Right lower. Same image showing co-immunofluorescence labelling for the Purkinje cell marker calbindin (magenta). Image from publication CC-BY-4.0. PMID: 37408217
Immunoblot against crude brain membranes from adult rat (RBM) and Kv1.1 wild-type (WT) or knock-out (KO) mouse probed with K36/15 (left) or N52A/42 (right) TC supe.
Representative confocal sections of lamina adjacent to the Purkinje cell layer from Kcna1V408A/+ and control cerebellar cortex, labelled for KV1.1 (Cat.75-105, 1:1000; yellow) and PSD-95 (centre, magenta). Right, merged image. Image from publication CC-BY-4.0. PMID: 37408217
Immunolabeling of postnatal 14 mouse CA1 (Axonal Initial Segment, Interneuron) specifically labeling extracellular Kv1.1 (Cat no 75-105, 1:200, green) and Ankyrin G (red). DNA labeled with DAPI. Mice transcardially perfused with cold 4% PFA in PBS, brain removed and postfixed overnight. 50 uM slices as floating sections, antigen retrieval performed with sodium citrate at 80 C for 30 min before immunolabeling. Images from: J. Ramirez-Franco and O. El Far, Aix-Marseille Université and INSERM.

Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

Go to Full Product Details

Data Sheet
KO Validated KO Validated

SKU: 75-105

Volume: 100 µL
Price:
$385.00
Ships: 1-2 business days

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
28115470not listedLang, B., et al. 2017. Intracellular and non-neuronal targets of voltage-gated potassium channel complex antibodies. Journal of Neurology, Neurosurgery & Psychiatry, 88(4), pp.353-361.. Journal of Neurology, Neurosurgery & Psychiatry, 353-361.
23774215not listedLallet-Daher, H., et al. 2013. Potassium channel KCNA1 modulates oncogene-induced senescence and transformation.. Cancer Research, 5253-5265.
206639771:100Irani, S.R., et al. 2010. Antibodies to Kv1 potassium channel-complex proteins leucine-rich, glioma inactivated 1 protein and contactin-associated protein-2 in limbic encephalitis, Morvan''s syndrome and acquired neuromyotonia.. Brain, 2734-2748.
Immunocytochemistry: Mouse
PMID Dilution Publication
283312031:100Rama, S., et al. 2017. The role of axonal Kv1 channels in CA3 pyramidal cell excitability. . Scientific reports, 1-12.
212242221:2000Karcz, A., et al. 2011. Low‐voltage activated Kv1. 1 subunits are crucial for the processing of sound source location in the lateral superior olive in mice. . The Journal of physiology, 1143-1157.
Immunocytochemistry: Rat
PMID Dilution Publication
331851901:100Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife, e56058.
33168717not listedCho, I.H., et al. 2020. The potassium channel subunit Kvβ1 serves as a major control point for synaptic facilitation. PNAS:USA, 29937-29947.
254477421:1000Hoppa, M.B., et al. 2014. Control and plasticity of the presynaptic action potential waveform at small CNS nerve terminals.. Neuron, 778-789.
238369291:1000Sosanya, N.M., et al. 2013. Degradation of high affinity HuD targets releases Kv1.1 mRNA from miR-129 repression by mTORC1.. The Journal of Biology, 53-69.
21357749not listedVacher, H., et al. 2011. Cdk-mediated phosphorylation of the Kvβ2 auxiliary subunit regulates Kv1 channel axonal targeting.. The Journal of Biology, 813-24.
17185748not listedVacher, H., et al. 2007. Regulation of Kv1 channel trafficking by the mamba snake neurotoxin dendrotoxin K.. The FASEB Journal, 906-914.
Immunohistochemistry: Mouse
PMID Dilution Publication
38663634not listedRamirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513.
38194969not listedMiyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
374082171:1000Martin, H, et al. 2023. Basket to Purkinje Cell Inhibitory Ephaptic Coupling Is Abolished in Episodic Ataxia Type 1. Cells, 1382.
372830581:750Dustin, E, et al. 2023. Adult-onset Depletion of Sulfatide Leads to Axonal Degeneration with Relative Myelin Sparing. Glia, 2285-2303.
368497161:100Hu, A., et al. 2023. Projection-Specific Heterogeneity of the Axon Initial Segment of Pyramidal Neurons in the Prelimbic Cortex. Neuroscience Bulletin, .
366409131:500Otuyemi, B., et al. 2023. Domain and Cell Type-specific Immunolocalisation of Voltage-gated Potassium Channels in the Mouse Striatum. Journal of Chemical Neuroanatomy, 102233.
362173001:200Nicholson, M., et al. 2022. Remodelling of Myelinated Axons and Oligodendrocyte Differentiation is Stimulated by Environmental Enrichment in the Young Adult Brain. The European Journal of Neuroscience, 6099-6114.
356618581:500Nascimento, A.I., et al. 2022. Sensory neurons have an axon initial segment that initiates spontaneous activity in neuropathic pain. Brain, 1632-1640.
317616701:200Klingseisen, A., et al. 2019. Oligodendrocyte neurofascin independently regulates both myelin targeting and sheath growth in the CNS. . Developmental cell, 730-744.
312487281:250Zheng, Y., et al. 2019. Deep sequencing of somatosensory neurons reveals molecular determinants of intrinsic physiological properties. . Neuron, 598-616.
294761051:200Nguyen, L.H., et al. 2018. mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy. Scientific Reports, 3568.
294761051:200Nguyen, L.H., et al. 2018. mTOR-dependent alterations of Kv1.1 subunit expression in the neuronal subset-specific Pten knockout mouse model of cortical dysplasia with epilepsy. Scientific Reports, 3568.
28134616not listedAmor, V., et al. 2017. The paranodal cytoskeleton clusters Na+ channels at nodes of Ranvier. . Elife, e21392.
25290690not listedJones, K.S., et al. 2014. Neonatal NMDA receptor blockade disrupts spike timing and glutamatergic synapses in fast spiking interneurons in a NMDA receptor hypofunction model of schizophrenia.. PLoS One, e109303.
247190871:100Desmazieres, A., et al. 2014. Differential stability of PNS and CNS nodal complexes when neuronal neurofascin is lost.. Journal of Neuroscience, 5083-5088.
242009241:40Liu, Q., et al. 2014. Heterogeneous intrinsic excitability of murine spiral ganglion neurons is determined by Kv1 and HCN channels.. Neuroscience, 96-110.
22612819not listedMenegola, M., et al. 2012. The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice.. Epilepsia, 142-149.
224737101:50Irani, S.R., et al. 2012. Morvan syndrome: clinical and serological observations in 29 cases.. Annals of Neurology, 241-255.
21958379not listedDuflocq, A., et al. 2011. Characterization of the axon initial segment (AIS) of motor neurons and identification of a para-AIS and a juxtapara-AIS, organized by protein 4.1B.. BMC Biology, 1-19.
21220016not listedGiacomini, C., et al. 2011. Both Schwann cell and axonal defects cause motor peripheral neuropathy in Ebf2-/- mice.. Neurobiology of Disease, 73-84.
191181651:1000Lorincz, A., et al. 2008. Cell-type-dependent molecular composition of the axon initial segment. . Journal of Neuroscience, 14329-14340.
190919621:1000Miller, M.N., et al. 2008. Region-specific spike-frequency acceleration in layer 5 pyramidal neurons mediated by Kv1 subunits.. Journal of Neuroscience, 13716-13726.
1846674910ug/mlGoldberg, E.M., et al. 2008. K+ channels at the axon initial segment dampen near-threshold excitability of neocortical fast-spiking GABAergic interneurons.. Neuron, 387-400.
Immunohistochemistry: Rat
PMID Dilution Publication
360502241:500Sharma, K., et al. 2022. Low-Voltage Activating K+ Channels in Cochlear Afferent Nerve Fiber Dendrites. Experimental Neurobiology, 243-259.
347994471:100Zbili, M., et al. 2021. Homeostatic regulation of axonal Kv1. 1 channels accounts for both synaptic and intrinsic modifications in the hippocampal CA3 circuit. PNAS:USA, .
323456731:300Housley, S.N., et al. 2020. Cancer exacerbates chemotherapy-induced sensory neuropathy. . Cancer research, 2940-2955.
283312031:50Rama, S., et al. 2017. The role of axonal Kv1 channels in CA3 pyramidal cell excitability. . Scientific reports, 1-12.
247232631:100Dufour, M.A., et al. 2014. Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development.. Journal of Neuroscience Research, 981-999.
246065841:250Kirizs, T., et al. 2014. Distinct axo-somato-dendritic distributions of three potassium channels in CA1 hippocampal pyramidal cells.. European Journal of Neuroscience, 1711-1783.
234391231:50Campanac, E., et al. 2013. Enhanced intrinsic excitability in basket cells maintains excitatory-inhibitory balance in hippocampal circuits.. Neuron, 712-722.
191181651:1000Lorincz, A., et al. 2008. Cell-type-dependent molecular composition of the axon initial segment. . Journal of Neuroscience, 14329-14340.
Immunoprecipitation: Mouse
PMID Dilution Publication
2206709915ug/mlBildl, W., et al. 2012. Extending the dynamic range of label-free mass spectrometric quantification of affinity purifications.. Molecular & Cellular Proteomics, .
2215851110ulLi, K.K., et al. 2011. Neuregulin 1 regulates excitability of fast-spiking neurons through Kv1.1 and acts in epilepsy.. Nature Neuroscience, 267-273.
Western Blot: Human
PMID Dilution Publication
23774215not listedLallet-Daher, H., et al. 2013. Potassium channel KCNA1 modulates oncogene-induced senescence and transformation.. Cancer Research, 5253-5265.
23734863not listedZhao, J., et al. 2013. Spinocerebellar ataxia-13 Kv3.3 potassium channels: arginine-to-histidine mutations affect both functional and protein expression on the cell surface.. Biochemical Journal, 259-265.
226096161:1000Zhu, J., et al. 2012. Characterization of the Kv1.1 I262T and S342I mutations associated with episodic ataxia 1 with distinct phenotypes.. Archives of Biochemistry and Biophysics, 99-105.
Western Blot: Mouse
PMID Dilution Publication
38663634not listedRamirez-Franco, J, et al. 2024. The downregulation of Kv1 channels in Lgi1-/-mice is accompanied by a profound modification of its interactome and a parallel decrease in Kv2 channels. Neurobiology of Disease, 106513.
38194969not listedMiyazaki, Y, et al. 2024. Oligodendrocyte-derived LGI3 and its receptor ADAM23 organize juxtaparanodal Kv1 channel clustering for short-term synaptic plasticity. Cell Reports, 113634.
25707359not listedChen, N., et al. 2015. Interaction proteomics of canonical Caspr2 (CNTNAP2) reveals the presence of two Caspr2 isoforms with overlapping interactomes.. Biochimica et Biophysica Acta, 827-833.
23945775not listedKyle, B.D., et al. 2013. The large conductance, calcium-activated K+ (BK) channel is regulated by cysteine string protein.. Scientific Reports, 2447.
22449950not listedBangratz, M., et al. 2012. A mouse model of Schwartz-Jampel syndrome reveals myelinating Schwann cell dysfunction with persistent axonal depolarization in vitro and distal peripheral nerve hyperexcitability when perlecan is lacking.. The American Journal of Pathology, 2040-2055.
220670991:10,000Bildl, W., et al. 2012. Extending the dynamic range of label-free mass spectrometric quantification of affinity purifications.. Molecular & Cellular Proteomics, .
Western Blot: Rat
PMID Dilution Publication
331851901:500Sanders, S.S., et al. 2020. The palmitoyl acyltransferase ZDHHC14 controls Kv1-family potassium channel clustering at the axon initial segment. Elife, e56058.
32437528not listedRamberger, M., et al. 2020. Distinctive binding properties of human monoclonal LGI1 autoantibodies determine pathogenic mechanisms. Brain, 1731-1745.
28673977not listedSeagar, M., et al. 2017. LGI1 tunes intrinsic excitability by regulating the density of axonal Kv1 channels. Proceedings of the National Academy of Sciences, 114(29), pp.7719-7724.. Proceedings of the National Academy of Sciences, 7719-7724.
252702941 : 500Sosanya, N.M., et al. 2015. Rapamycin reveals an mTOR-independent repression of Kv1.1 expression during epileptogenesis.. Neurobiology of Disease, 96-105.
17185748not listedVacher, H., et al. 2007. Regulation of Kv1 channel trafficking by the mamba snake neurotoxin dendrotoxin K.. The FASEB Journal, 906-914.

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