Anti-Kir2.2 K+ Channel Antibody (N124B/38)

Name: Anti-Kir2.2 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-203
Price: 329.0 USD
Availability: InStock

6 Citations

Our Anti-Kir2.2 K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N124B/38. It detects rat Kir2.2 K+ channel, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.2 or untagged Kv2.1 plasmids and probed with N124B/38 TC supe.

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Species Reactivity

Rat

Applications

ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-203

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Kir2.2 K+ channel

Target Description

ATP-sensitive inward rectifier potassium channel 12or Kir2.2 is encoded by the gene KCNJ12. Kir2.2 is a member of the inward rectifier potassium ion channel family and plays a role controlling the resting membrane potential in muscle cells and neurons. Kir2.2 is an integral membrane protein that is expressed in heart, brain and in aortic endothelial cells. In brain, Kir2.2 is expressed throughout the forebrain and cerebellum. Diseases associated with KCNJ12 include Smith-Magenis Syndrome and Leber Congenital Amaurosis 16.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N124B/38

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Kcnj12 Irk2

Molecular Weight

48 kDa

Antigen

Synthetic peptide amino acids 390-410 (cytoplasmic C-terminus) of rat Kir2.2 (accession number P52188)

Species Reactivity

Rat

Antibody Registry ID

AB_2130486

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity against Kir2.1 or Kir2.3

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

ATP-sensitive inward rectifier potassium channel 12 (Inward rectifier K(+) channel Kir2.2) (IRK-2) (Potassium channel, inwardly rectifying subfamily J member 12)

UniProt ID

P52188

Product Specific References for Applications and Species

Immunocytochemistry: Mouse

PLA: Rat

Western Blot: Mouse | Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
29802197	1:50	Cheng, L., et al. 2018. Coupling of Smoothened to inhibitory G proteins reduces voltage-gated K+ currents in cardiomyocytes and prolongs cardiac action potential duration. . Journal of Biological Chemistry, 11022-11032.
21507952	1:50 (supe)	Cheng, L., et al. 2011. Cortactin is required for N-cadherin regulation of Kv1.5 channel function.. The Journal of Biological Chemistry, 20478-20489.

PLA: Rat
PMID	Dilution	Publication
36060074	1:200	Xu, Y., et al. 2022. Hypoxia Inhibits the Cardiac I Current Through SUMO Targeting Kir2.1 Activation by PIP2. iScience, 104969.

Western Blot: Mouse
PMID	Dilution	Publication
26611208	not listed	Zhang, X., et al. 2016. Persistent increases in Ca(2+) influx through Cav1.2 shortens action potential and causes Ca(2+) overload-induced afterdepolarizations and arrhythmias.. Basic Research in Cardiology, 45307.
25545278	1:500	DiFranco, M., et al. 2015. Inward rectifier potassium currents in mammalian skeletal muscle fibres.. The Journal of Physiology, 1213-1238.

Western Blot: Rat
PMID	Dilution	Publication
20530486	not listed	Vaidyanathan, R., et al. 2010. Regulation of cardiac inward rectifier potassium current (I(K1)) by synapse-associated protein-97.. The Journal of Biological Chemistry, .

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Anti-Kir2.2 K+ Channel Antibody (N124B/38)

SKU: 75-203

Product Details

Product Specific References for Applications and Species

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