Anti-Ki-67 Antibody (6B4)

Our Anti-Ki-67 mouse monoclonal primary antibody detects human Ki-67, and is affinity-purified. It is validated for use in ICC, WB.

Left: Immunofluorescence analysis of HeLa cells costained with mouse antibody to Ki-67 (1:2,000, red) and chicken antibody to vimentin (C-1409-50, 1:10,000, green). Ki-67 protein is predominantly localized in nucleoli of _cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosome. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates probed with mouse antibody to Ki-67 (1:2,000, Lanes 2 and 3). Two bands above 250 kDa are observed, corresponding to major Ki-67 isoforms of molecular weight ~345 and 395 kDa.
Analysis of Ki67 expression (green) in cultured HeLa cells with mouse anti-Ki67 antibody by Immunocytochemistry. The nuclei of rapidly dividing cells have clumps of Ki67 associated with nucleoli, while nearby quiescent cells express little or no Ki67. Cells are counterstained with chicken anti-lamin A/C antibody (C-1698-100, red) which reveals the nuclear lamina. Blue: DAPI nuclear stain.
Left: Immunofluorescence analysis of HeLa cells costained with mouse antibody to Ki-67 (1:2,000, red) and chicken antibody to vimentin (C-1409-50, 1:10,000, green). Ki-67 protein is predominantly localized in nucleoli of _cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosome. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates probed with mouse antibody to Ki-67 (1:2,000, Lanes 2 and 3). Two bands above 250 kDa are observed, corresponding to major Ki-67 isoforms of molecular weight ~345 and 395 kDa.
Left: Immunofluorescence analysis of HeLa cells costained with mouse antibody to Ki-67 (1:2,000, red) and chicken antibody to vimentin (C-1409-50, 1:10,000, green). Ki-67 protein is predominantly localized in nucleoli of _cells in interphase, while in mitotic cells Ki-67 is associated with condensed chromosome. Ki-67 is not expressed in cells in quiescent G0 state. Blue: DAPI staining of nuclear DNA. Right: Western blot analysis of equal amounts of HeLa (Lane 2) and HEK293 (Lane 3) cell lysates probed with mouse antibody to Ki-67 (1:2,000, Lanes 2 and 3). Two bands above 250 kDa are observed, corresponding to major Ki-67 isoforms of molecular weight ~345 and 395 kDa.
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Human
ICC, WB
Mouse

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Data Sheet

SKU: M-1805-100

Volume/Size: 100 µg
Price:
$327.00

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