Anti-Ki-67 Antibody (6G3)

Name: Anti-Ki-67 Antibody
Brand: PhosphoSolutions
SKU: 1007-Ki67
Price: 456.0 USD
Availability: InStock

Our Anti-Ki-67 Antibody primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Ki-67 and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunostaining of HeLa cells showing specific labeling of Ki-67 in the nucleoli of rapidly dividing cells, (1:2000) in red. DNA is stained blue.

Western blot of dividing HeLa cell cultures (2) and HEK293 cell cultures (3) showing specific immunolabeling of the ~345/395 Ki-67 isoforms.

Immunostaining of HeLa cells showing specific labeling of Ki-67 in the nucleoli of rapidly dividing cells, (1:2000) in red. DNA is stained blue.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: 1007-Ki67

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Ki-67

Scientific Background

Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).

Format

Protein G Purified

Clonality

Monoclonal

Clone

6G3

Isotype

IgG1

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

MKI67

Molecular Weight

345-395 kDa

Immunogen

Recombinant construct containing the N-terminal 300 amino acids of the human sequence expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human

Antibody Registry ID

AB_2905461

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatent

Buffer

PBS + 50% glycerol and 5 mm NaN₃

Dilution Ranges

WB: 1:2000
ICC: 1:2000-1:5000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.

Quality Control

Western blots performed on each lot

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Antigen identified by monoclonal Ki 67; Antigen identified by monoclonal Ki-67; Antigen KI-67; Antigen KI67; Ki67; KI67_HUMAN; KIA; Marker of proliferation Ki-67; MIB 1; MIB; MKI67; PPP1R105; Proliferation marker protein Ki-67; Proliferation related Ki 67 antigen; Protein phosphatase 1 regulatory subunit 105; RP11-380J17.2

UniProt ID

P46013
UniProt Summary: Protein that associates with the surface of mitotic chromosomes and acts both as a chromosome repellent during early mitosis and chromosome attractant during late mitosis (PubMed:27362226, PubMed:32879492, PubMed:35513709, PubMed:39153474). Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). During early mitosis, relocalizes from nucleoli to the chromosome surface where it forms extended brush structures that cover a substantial fraction of the chromosome surface (PubMed:27362226). The MKI67 brush structure prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). During mitotic anaphase, the MKI67 brush structure collapses and MKI67 switches from a chromosome repellent to a chromosome attractant to promote chromosome clustering and facilitate the exclusion of large cytoplasmic particles from the future nuclear space (PubMed:32879492, PubMed:39153474). Mechanistically, dephosphorylation during mitotic exit and simultaneous exposure of a conserved basic patch induce the RNA-dependent formation of a liquid-like condensed phase on the chromosome surface, promoting coalescence of neighboring chromosome surfaces and clustering of chromosomes (PubMed:39153474). Binds premature ribosomal RNAs during anaphase; promoting liquid-liquid phase separation (PubMed:28935370, PubMed:39153474). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization; it is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in mitotic chromosome (PubMed:24867636). {ECO:0000250|UniProtKB:E9PVX6, ECO:0000269|PubMed:10878551, ECO:0000269|PubMed:24867636, ECO:0000269|PubMed:27362226, ECO:0000269|PubMed:28935370, ECO:0000269|PubMed:32879492, ECO:0000269|PubMed:35513709, ECO:0000269|PubMed:39153474}.

NCBI Gene ID

4288

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Anti-Ki-67 Antibody (6G3)

SKU: 1007-Ki67

Product Details

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