Our Anti-Ki-67 Antibody primary antibody from PhosphoSolutions is mouse monoclonal. It detects human Ki-67 and is Protein G purified. It is great for use in WB, IHC, ICC.
Immunostaining of HeLa cells showing specific labeling of Ki-67 in the nucleoli of rapidly dividing cells, (1:2000) in red. DNA is stained blue.
Ki-67 is a nuclear protein that is considered to be essential for cellular proliferation since it is expressed only in active phases of the cell cycle (G1, S, G2 and mitosis) but not in resting phase G0 (Scholzen & Gerdes 2000). Ki-67 antibodies have therefore been widely used as markers of cell growth. Ki-67 has further been identified as a predicative and prognostic marker in breast, lung, cervical and other cancers (Yerushalmi et al., 2010; Folescu et al., 2018; Das et al., 2018).
Protein G Purified
Monoclonal
6G3
IgG1
ICC, WB
Mouse
MKI67
345-395 kDa
Recombinant construct containing the N-terminal 300 amino acids of the human sequence expressed in and purified from E. coli.
Human
Human
AB_2905461
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified culture supernatent
PBS + 50% glycerol and 5 mm NaN3
WB: 1:2000
ICC: 1:2000-1:5000
Unconjugated
Specific for endogenous levels of the ~345 & 395 kDa Ki-67 isoforms. Additional bands are thought to be breakdown products of these 2 isoforms.
Western blots performed on each lot
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
