Our Anti-KCNU1/Slo3 pH-sensitive maxi-K+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N2/16. It is KO validated, detects mouse, Xenopus KCNU1/Slo3 pH-sensitive maxi-K+ channel, and is purified by Protein A chromatography. It is great for use in EM, ICC, IP, WB.
Immunoblot versus (E) membrane proteins from wild- type (wt), Slo3-eGFP and Slo3 knockout (Slo3-/-) mouse testes and (F) immunoprecipitation products from total testes proteins pulled down with N2/16 probed with N2/16. Data courtesy of Christopher Lingle (Washington University) and reproduced with permission from the Proceedings of the National Academy of Sciences of the United States of America (2011 Zeng et al., PMID 21427226).
Potassium calcium-activated channel subfamily U member 1 is encoded by the gene KCNU1. KCNU1 is a member of the potassium channel family, calcium-activated (TC 1.A.1.3) subfamily, KCa5.1/KCNU1 sub-subfamily. KCNU1 is a testis-specific potassium channel activated by both intracellular pH and membrane voltage that mediates transport of K+. KCNU1 is expressed in the testis. Diseases associated with KCNU1 include Leukodystrophy, Hypomyelinating, 3 and Coffin-Siris Syndrome 1.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N2/16
IgG1
EM, ICC, IP, WB
Mouse
Kcnu1 Kcnma3 Ksper Slo3
115 kDa
Fusion protein amino acids 1052-1121 of mouse Slo3 (accession number O54982) produced recombinantly in E. Coli
Gerbil, Mouse
AB_2131864
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4
WB: 1:500
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Shipped on ice packs
Potassium channel subfamily U member 1 (Calcium-activated potassium channel subunit alpha-3) (Calcium-activated potassium channel, subfamily M subunit alpha-3) (Pore-forming subunit of the sperm-specific alkalization activated K(+) current) (KSper) (Slowpoke homolog 3) (mSlo3) (pH-sensitive maxi potassium channel)
Yang, C., et al. 2011. LRRC52 (leucine-rich-repeat-containing protein 52), a testis-specific auxiliary subunit of the alkalization-activated Slo3 channel.. PNAS: USA, 19419-19424.
Yang, C., et al. 2011. LRRC52 (leucine-rich-repeat-containing protein 52), a testis-specific auxiliary subunit of the alkalization-activated Slo3 channel.. PNAS: USA, 19419-19424.
Zeng, X.H., et al. 2015. SLO3 auxiliary subunit LRRC52 controls gating of sperm KSPER currents and is critical for normal fertility.. PNAS: USA, 2599-2604.
Yang, C.T., et al. 2009. Interactions between beta subunits of the KCNMB family and Slo3: beta4 selectively modulates Slo3 expression and function.. PLoS One, e6135.
Martínez-López, P., et al. 2009. Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by Slo3 channels.. Biochemical and Biophysical Research Communications, 204-209.
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