Anti-Integrin alpha-M (CD11b, MAC-1, OX-42 Antigen) Antibody

Name: Anti-Integrin alpha-M (CD11b, MAC-1, OX-42 Antigen) Antibody
Brand: Aves Labs
SKU: MAC
Price: 440.0 USD
Availability: InStock

from Aves Labs Datasheet

1 Citation

Our anti-Integrin Alpha-M (MAC-1) antibody was purified from eggs collected from chickens injected with a KLH-conjugated peptide corresponding to a sequence shared between the mouse (NP_001076429.1) and human gene products (NP_001139280.1). These antibodies have been validated with human, mouse and rat tissues.

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

Format

Affinity-Purified IgY

Gene Name

ITGAM CD11B CR3A

Molecular Weight

170 kDa

Go to Full Product Details

Low-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).

Higher-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).

Frame showing MAC-1 immunoreactivity in human microglial cells in an Alzheimer's Disease patient. Photomicrograph taken by Dr. Randy Woltjer, Pathology, OHSU.

Low-power view of MAC1 immunoreactivity in microglial cells in a paraformaldehyde (4%)-fixed paraffin-embedded section through cerebral cortex of an adult mouse brain. 1˚ antibody was Aves Labs' anti-MAC1 antibody (1:2000 dilution); 2˚ antibody was a Texas Red goat anti-chicken IgY (Jackson ImmunoResearch; 1:1000 dilution).

Volume: 1000 µL

Variant

Price:

$440

Quantity:

Bulk Order Anti-Integrin alpha-M (CD11b, MAC-1, OX-42 Antigen) Antibody

Product Details

Target

Integrin Alpha M

Target Description

Integrin Alpha M (also known as MAC-1 and CD11b) is a 1142 amino acid protein expressed in various antigen-presenting cells of the immune system, including microglial cells of the central nervous system. This is the same protein that is recognized by the Ox-42 mouse monoclonal antibody.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

ITGAM CD11B CR3A

Molecular Weight

170 kDa

Antigen

Synthetic peptide

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313548

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Hens were immunized with a Keyhole Limpet Hemocyanin (KLH)-conjugate of a synthetic peptide corresponding to a region near the center of the extracellular domain of this single-membrane pass gene product). After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached. Finally, the antibody concentration was adjusted to 100 µg/mL and filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:2000-1:5000
IHC: 1:2000-1:5000
ICC: 1:2000-1:5000

Quality Control

Antibodies were analyzed by immunohistochemistry (at a concentration of 3 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) or HRP-labeled goat anti-chicken IgY (1:2000 dilution, Aves Labs, Cat.# H-1004) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Shipping

Ice packs

Synonyms

Integrin alpha-M (CD11 antigen-like family member B) (CR-3 alpha chain) (Cell surface glycoprotein MAC-1 subunit alpha) (Leukocyte adhesion receptor MO1) (Neutrophil adherence receptor) (CD antigen CD11b)

UniProt Details

UniProt (Immunogen Species): P11215

Merlo S, Spampinato SF, Beneventano M, Sortino MA. (2018), 'The contribution of microglia to early synaptic compensatory responses that precede β-amyloid-induced neuronal death.' Nature. 10.1038/s41598-018-25453-1.

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Anti-Integrin alpha-M (CD11b, MAC-1, OX-42 Antigen) Antibody