Anti-iNOS (C-terminal region) Antibody (M398)

Name: Anti-iNOS (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: NM3981
Price: 292.0 USD
Availability: InStock

Our iNOS (C-terminal region) primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat iNOS (C-terminal region) and is Protein A purified. It is great for use in WB, ICC.

Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1µg/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NP2131).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Mouse

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Data Sheet

SKU: NM3981

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

iNOS (C-terminal region)

Scientific Background

Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M398

Isotype

IgG2a

Applications

ELISA, WB

Host Species

Mouse

Gene Name

NOS2

Molecular Weight

130

Immunogen

Clone (M398) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human iNOS. The human iNOS sequence used has high homology with similar regions in rat and mouse iNOS.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:250
ICC: 1:50

Conjugate

Unconjugated

Specificity

The antibody detects a 130 kDa* protein on SDS-PAGE immunoblots of mouse macrophages (RAW264.7) treated with IFNγ and LPS, or J774A.1 cells treated with LPS only.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

HEP-NOS; Nos2 nitric oxide synthase 2; NOS2A; nitric oxide synthase; inducible; NOS; type II; hepatocyte NOS; inducible NO synthase; inducible NOS; nitric oxide synthase 2; nitric oxide synthase 2A (inducible; hepatocytes; macrophage; peptidyl-cysteine S-nitrosylase NOS2; NOSII; Hepatocyte

UniProt ID

P35228
UniProt Summary: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH on 'Cys-247' implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.

NCBI Gene ID

4843

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Anti-iNOS (C-terminal region) Antibody (M398)

SKU: NM3981

Product Details

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