Anti-Iba1/AIF1 Antibody

2 Citations

Our Anti-IBA1/AIF1 chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects human, mouse, and rat IBA1/AIF1, and is affinity purified. It is great for use in ICC, WB.

Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
Immunocytochemistry of iPSC-derived microglial cultures labelling Iba1 marker (Cat no IBA1-0100, 1:500, red), scale bar = 275 μm. Image kindly provided by Patricia Villegas Zafra, Achucarro Basque Center for Neuroscience.
Immunostaining of 5xFAD mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of IBA1 positive cells (cat. IBA1, 1:100; green) in the cortex. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.
Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
Immunofluorescence of AIF1/IBA1-FLAG transfected COS-7 cells using chicken α-AIF1/IBA1 (green) and mouse α-flag tag (red). Yellow/orange staining shows 100% correspondence between the two antibodies for recognition of transfected cells. Blue staining is DAPI and stains nuclei of both transfected and untransfected cells.
Western blot of rat brain membrane lysate using chicken α-AIF1/IBA1 showing specific immunolabeling of endogenous AIF1/IBA1 at ~17kDa.
Immunocytochemistry of iPSC-derived microglial cultures labelling Iba1 marker (Cat no IBA1-0100, 1:500, red), scale bar = 275 μm. Image kindly provided by Patricia Villegas Zafra, Achucarro Basque Center for Neuroscience.
Immunostaining of 5xFAD mouse brain section fixed in 4% paraformaldehyde and preserved in cryoprotectant showing specific detection of IBA1 positive cells (cat. IBA1, 1:100; green) in the cortex. Nuclei stained with DAPI (blue). Background stain was attenuated with TrueVIEW. Image kindly provided by Ifeoluwa (Hiphy) Awogbindin of the Tremblay Lab, Division of Medical Sciences, University of Victoria, Canada.

ICC, WB
Chicken

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Data Sheet
Trial Size Trial Size

SKU: IBA1-0100

Volume: 500 µL
Price:
$135.00

Product Specific References for Applications and Species

  • Immunohistochemistry: Mouse
Immunohistochemistry: Mouse
PMID Dilution Publication
36405424not listedCarpenter, J.M., et al. 2022. Evaluation of Delayed LNFPIII Treatment Initiation Protocol on Improving Long-Term Behavioral and Neuroinflammatory Pathology in a Mouse Model of Gulf War Illness. Brain, Behavior, & Immunity, 100553.
35633501not listedGalloway, D.A., et al. 2022. Investigating the NLRP3 Inflammasome and its Regulator miR-223-3p in Multiple Sclerosis and Experimental Demyelination. Journal of Neurochemistry, 94-112.

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