Our Anti-Hsp70 (Ser153) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human Hsp70 (Ser153) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of T47D cell lysate treated with EGF (1 nM) for 60 minutes showing specific labeling of the ~70 kDa Hsp70 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is eliminated or significantly decreased with lambda phosphatase (λ-Ptase, 1200 units for 30 minutes).
The Hsp70 family of heat shock proteins are considered stress-induced survival proteins as they are expressed when exposed to factors such as heat, hypoxia, oxidative stress, altered pH or by underlying factors like cancer (Daugaard et al. 2007). Hsp70 proteins are able to bind hydrophobic residues on misfolded proteins, thereby preventing their aggregation and thus serving important roles in protein homeostasis. To date, there have been 8 members of the family identified; the majority of which are found in the cytosol though some have specific function in tissue or an organelle (Murphy, 2013). Hsp70 has also been shown to play a major role in cancer; from tumor grade to prognosis, as well as chemotherapeutic drug resistance (Ciocca et al, 2005). There have been several phospho-serine and threonine sites identified within Hsp70, the role of each one has yet to be determined.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
HSPA1A
70 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr153 of human HSP, conjugated to keyhole limpet hemocyanin (KLH).
Human
AB_2636998
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for the ~70 kDa Hsp70 phosphorylated at Ser153. Immunolabeling of Hsp70 is completely eliminated with λ-phosphatase treatment. Additional bands detected are thought to be other members of the Hsp70 family.
Phosphorylated
Ser153
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DnaK type molecular chaperone HSP70 1 antibody, Epididymis secretory protein Li 103 antibody, FLJ54303 antibody, FLJ54370 antibody, FLJ54392 antibody, FLJ54408 antibody, FLJ75127 antibody, Heat shock 70 kDa protein 1 antibody, Heat shock 70 kDa protein 1/2 antibody, Heat shock 70 kDa protein 1A antibody, Heat shock 70 kDa protein 1A/1B antibody, Heat shock 70kDa protein 1B antibody, Heat shock induced protein antibody, HEL S 103 antibody, HSP70 1 antibody, HSP70 1A antibody, HSP70 1B antibody, HSP70 2 antibody, HSP70-1 antibody, HSP70-1/HSP70-2 antibody, HSP70-1A antibody, HSP70.1 antibody, HSP70.1/HSP70.2 antibody, HSP71_HUMAN antibody, HSP72 antibody, HSPA1 antibody, HSPA1A antibody, HSPA1B antibody
Daugaard M, Rohde M, Jäättelä M (2007) The Heat Shock Protein 70 Family:Highly homologous proteins with overlapping and distinct functions. FEBS Lett, 581: 3702-10. PMID: 17544402
Ciocca DR, Calderwood SK (2005) Heat Shock Proteins in Cancer: Diagnostic, Prognostic, Predictive, and Treatment Implications. Cell Stress Chaperones, 10: 86-103. PMID: 16038406
Murphy ME (2013) The Hsp70 Family and Cancer. Carcinogenesis, 34:1181-8. PMID: 23563090
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