Anti-Histone H2B (Ser-36), Phosphospecific Antibody
Our Histone H2B (Ser-36) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Histone H2B (Ser-36) and is antigen affinity purified. It is great for use in WB.
Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B (C-terminus) (lanes 1 & 2), anti-Histone H2B (Ser-36) (lanes 3 & 4), and anti-Histone H2B (a.a. 33-47) (lanes 5 & 6).
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Product Specific References for Applications and Species
| Immunohistochemistry: Mouse | ||
| PMID | Dilution | Publication |
| 34745014 | 1:100 | Faure, MC, et al. 2021. In Utero Exposure to Metformin Reduces the Fertility of Male Offspring in Adulthood. Frontiers in endocrinology, 750145. |
| Western Blot: Human | ||
| PMID | Dilution | Publication |
| 27151441 | not listed | Yi, S.A., et al. 2016. S6K1 Phosphorylation of H2B Mediates EZH2 Trimethylation of H3: A Determinant of Early Adipogenesis. Molecular Cell, 443-452. |
| 27149922 | 1:200-1:1000 | Li, J., et al. 2016. Nuclear PKC-θ facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. Journal of Cell Science, 2448-2461. |
| 25813038 | not listed | Lee, J.H., et al. 2015. AKT phosphorylates H3-threonine 45 to facilitate termination of gene transcription in response to DNA damage. Nucleic Acids Research, 4505-4516. |
| 25533949 | not listed | Sajish, M., et al. 2015. A human tRNA synthetase is a potent PARP1-activating effector target for resveratrol. Nature, 370-373. |
| Western Blot: Insect | ||
| PMID | Dilution | Publication |
| 24036077 | not listed | Soe, K.C., et al. 2013. Transcriptional coactivator CIITA, a functional homolog of TAF1, has kinase activity. Biochimica et Biophysica Acta, 1184-1190. |
| Western Blot: Mouse | ||
| PMID | Dilution | Publication |
| 32822587 | not listed | Huang, D, et al. 2020. Functional Interplay between Histone H2B ADP-Ribosylation and Phosphorylation Controls Adipogenesis. Molecular cell, 934-949. |
| 30316515 | not listed | Nam, K.H., et al. 2018. Eudesmin impairs adipogenic differentiation via inhibition of S6K1 signaling pathway. Biochemical and Biophysical Research Communications, 1148-1153. |
| 26772142 | not listed | Bertoldo, M.J., et al. 2016. Specific deletion of AMP-activated protein kinase (α1AMPK) in mouse Sertoli cells modifies germ cell quality. Molecular and Cellular Endocrinology, 96-112. |
| Western Blot: Roundworm | ||
| PMID | Dilution | Publication |
| 28289190 | not listed | Demoinet, E, et al. 2017. AMPK blocks starvation-inducible transgenerational defects in Caenorhabditis elegans. PNAS USA, E2689-E2698. |