Anti-His-Tag Antibody

Name: Anti-His-Tag Antibody
Brand: Biosensis
SKU: R-1312-100
Price: 407.0 USD
Availability: InStock

Our Anti-His-Tag rabbit polyclonal primary antibody detects His-Tag, and is affinity-purified. It is validated for use in ELISA, ICC, IHC-Frozen, WB.

Western Blot analysis of Rabbit polyclonal anti-HIS tag R-1312-100. Lane 1 contains control 293 cells. Lane 2 contains 293 cells transfected with His-tagged S6. Lane 3 contains 293 cells transfected with His-tagged CREB.

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Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1312-100

Volume/Size: 100 µg

Variant

Price:

$407.00

Quantity:

Product Details

Target

His-Tag

Scientific Background

The 6X His tag is a short peptide sequence of 6 histidine residues. Epitopes such as the 6X His tag are often included with the target DNA at the time of cloning to produce fusion proteins containing the tag sequence. This allows anti-epitope tag antibodies such as this one to serve as a universal detection reagent for any recombinant protein containing this tag. Anti-epitope antibodies are a useful alternative to generating antibodies to identify a specific recombinant protein. The 6X His motif is often used as a tag on recombinant proteins to facilitate purification with immobilized metal-affinity chromatography.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, ICC, IHC, WB

Host Species

Rabbit

Immunogen

The antiserum was produced by immunizing rabbit with synthesized peptide containing 6 Histidine residues.

Immunogen Species

Species Independent

Storage

Stable for 1 year at -20°C from the date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing.

Physical State

Liquid

Buffer

PBS, pH 7.4 with 0.02% sodium azide.

Dilution Ranges

WB: 1:500-1:1000
IHC: 5 µg/mL
ICC: 5 µg/mL
IP: 5 µg/sample
ELISA: 1:2500

Conjugate

Unconjugated

Specificity

His-tagged fusion proteins.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Shipping

2-8°C (on cold packs)

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Anti-His-Tag Antibody

SKU: R-1312-100

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