Anti-HDAC2 (Ser394) Antibody
Our Anti-HDAC2 (Ser394) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat HDAC2 (Ser394) and is antigen affinity purified from pooled serum. It is great for use in WB.
SKU: p1180-394
Product Details
HDAC2 (Ser394)
Histone Deacetylase 2 (HDAC2) is part of a family of histone deacetylases that are responsible for deacetylation of lysine residues in the histone core. HDAC2 is classified as a class I histone deacetylase and is ubiquitously expressed throughout the body (Kee et al, 2008). It has been shown that HDAC2 plays an important role in cardiac hypertrophy (Eom et al, 2011). Phosphorylation of Ser-394 is responsible for the hypertrophy-associated activation of HDAC2, whereas intrinsic basal activity is maintained by phosphorylation of Ser-422 and Ser-424 (EOM et al, 2011).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
HDAC2
55 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser394 of human HDAC2, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Chicken, Guinea Pig, Human, Non-Human Primate, Sheep
AB_2492136
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~55 kDa HDAC2 protein phosphorylated at Ser394. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser394
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
D10Wsu179e antibody, HD 2 antibody, HD2 antibody, HDAC 2 antibody, Hdac2 antibody, HDAC2_HUMAN antibody, Histone deacetylase 2 (HD2) antibody, Histone deacetylase 2 antibody, OTTHUMP00000017046 antibody, OTTHUMP00000227077 antibody, OTTHUMP00000227078 antibody, RPD3 antibody, transcriptional regulator homolog RPD3 antibody, YAF1 antibody, YY1 associated factor 1 antibody, YY1 transcription factor binding protein antibody, Yy1bp antibody
UniProt (Human): Q92769
UniProt (Immunogen Species): Q92769
UniProt (Immunogen Species): Q92769
3066
Product Specific References for Applications and Species
- Western Blot: Mouse
Western Blot: Mouse | ||
PMID | Dilution | Publication |
32138755 | 1:1000 | Buck, J.M., et al. 2020. Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice. Epigenetics and Chromatin, 13(1), pp.1-15. |
Product Specific Protocols
- Western Blot Protocol: Download