Anti-Green Fluorescent Protein Tag (GFP) Antibody

Name: Anti-Green Fluorescent Protein Tag (GFP) Antibody
Brand: Biosensis
SKU: G-2103-100
Price: 337.0 USD
Availability: InStock

Our Anti-Green fluorescent protein (GFP) goat polyclonal primary antibody detects Green fluorescent protein (GFP), and is affinity-purified. It is validated for use in ICC, WB.

Left: Analysis of GFP fusion protein-transfected HEK293 cells by Immunocytochemistry. GFP expression (green) was detected with goat antibody to GFP (red, 1:5,000). Blue: Hoechst nuclear stain. The goat antibody reveals GFP protein expressed only in transfected cells, and as a result these cells appear yellow in color. Top: merged image; bottom panel: individual fluorescence channels. Right: Western blot analysis of GFP expression (green) in transfected HEK293 cells with goat anti-GFP antibody (1:1,000). Lane 1: molecular weight marker; Lane 2: non-transfected control cells; Lane 3: GFP-transfected cells; Lane 4: mCherry-transfected cells. A strong band at ~27 kDa corresponds to GFP protein detected only in cells transfected with GFP construct. This antibody does not recognize the mCherry protein.

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Applications

ICC, WB

Host Species

Goat

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Data Sheet

SKU: G-2103-100

Volume/Size: 100 µg

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Green fluorescent protein (GFP)

Scientific Background

The green fluorescent protein (GFP) is a 27 kDa protein isolated originally from the jellyfish Aequoria victoria. It has an endogenous fluorochrome activity with excitation maximum at 395 nm and emission maximum at 509 nm, which is similar to that of fluorescein. GFP can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.
This GFP rabbit antibody was made against a recombinant GFP construct originating from an Aequoria species which was engineered to improve spectral properties and prevent oligomerization. This form of GFP, referred to as AcGFP, is 94% identical to the eGFP developed by Tsien and co-workers. The antibody can be used to verify the expression, size and stability of both AcGFP and eGFP fusion proteins in western blotting experiments and to amplify GFP signals in tissues of transgenic animals.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Goat

Molecular Weight

27 kDa

Immunogen

Recombinant AcGFP protein expressed in and purified from E. Coli.

Immunogen Species

Jellyfish

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks). Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:1000-1:5000
ICC: 1:2000-1:5000

Conjugate

Unconjugated

Specificity

Antibody recognizes GFP protein in GFP-transfected HEK293 cells, but not in non-transfected control cells. Specific for GFP, does not cross-react with mCherry.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Green fluorescent protein; GFP

UniProt ID

Q6YGZ0

Shipping

25°C (ambient)

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Anti-Green Fluorescent Protein Tag (GFP) Antibody

SKU: G-2103-100

Product Details

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