Anti-Grb2 Antibody (M331)

Name: Anti-Grb2 Antibody
Brand: PhosphoSolutions
SKU: GM3311
Price: 379.0 USD
Availability: InStock

Our Grb2 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human, mouse, and rat Grb2 and is Protein A purified. It is great for use in WB, ICC, IP.

Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

Immunocytochemical labeling of Grb2 in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal Grb2 (GM3311) antibody, then detected using appropriate secondary antibody conjugated to Cy3.

Western blot of rat PC12 cell lysate. The blot was probed with mouse monoclonal anti-Grb2 antibody at 1:250 (lane 1), 1:500 (lane 2), or 1:1000 (lane 3).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Go to Full Product Details

Data Sheet

SKU: GM3311

Volume: 100 µL

Variant

Price:

$379.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Grb2

Scientific Background

Crk and Grb2 family adaptor proteins are involved in a variety of cell signaling pathways related to human diseases. These adaptors have Src homology 2 (SH2) and Src homology 3 (SH3) domains, which are docking sites for several signaling proteins, including receptors, kinases, and GTPase regulators. In addItion, other protein-protein interactions may also utilize Crk and Grb2 domain interactions to modulate cell signaling pathways. The DOCK family of proteins (DOCK3, DOCK4, and DOCK5) can interact with Crk and may be important Crk effector proteins. The cell cycle regulator p27Kip1 can interact with Grb2, and this implicates Grb2 activity in cell signaling pathways that alter cell cycle progression.

Format

Protein A Purified

Clonality

Monoclonal

Clone

M331

Isotype

IgG1

Applications

ELISA, ICC, IP, WB

Host Species

Mouse

Gene Name

GRB2

Molecular Weight

Immunogen

Clone (M331) was generated from a full length recombinant rat Grb2 protein. This sequence has high homology to human and mouse Grb2.

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:50
IP: 1:50

Conjugate

Unconjugated

Specificity

This antibody detects a 24 kDa* protein corresponding to the molecular mass of Grb2 on SDS-PAGE immunoblots of rat PC12.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Ash-psi; Grb; SH2/SH3 adapter GRB2; adapter protein GRB2; protein Ash

UniProt ID

P62994
UniProt Summary: Non-enzymatic adapter protein that plays a pivotal role in precisely regulated signaling cascades from cell surface receptors to cellular responses, including signaling transduction and gene expression. Thus, participates in many biological processes including regulation of innate and adaptive immunity, autophagy, DNA repair or necroptosis. Controls signaling complexes at the T-cell antigen receptor to facilitate the activation, differentiation, and function of T-cells. Mechanistically, engagement of the TCR leads to phosphorylation of the adapter protein LAT, which serves as docking site for GRB2. In turn, GRB2 establishes a a connection with SOS1 that acts as a guanine nucleotide exchange factor and serves as a critical regulator of KRAS/RAF1 leading to MAPKs translocation to the nucleus and activation. Functions also a role in B-cell activation by amplifying Ca(2+) mobilization and activation of the ERK MAP kinase pathway upon recruitment to the phosphorylated B-cell antigen receptor (BCR). Plays a role in switching between autophagy and programmed necrosis upstream of EGFR by interacting with components of necrosomes including RIPK1 and with autophagy regulators SQSTM1 and BECN1. Regulates miRNA biogenesis by forming a functional ternary complex with AGO2 and DICER1. Functions in the replication stress response by protecting DNA at stalled replication forks from MRE11-mediated degradation. Mechanistically, inhibits RAD51 ATPase activity to stabilize RAD51 on stalled replication forks. Additionally, directly recruits and later releases MRE11 at DNA damage sites during the homology-directed repair (HDR) process.

NCBI Gene ID

81504

Shipping

Blue Ice

Recover password

Create my account

Anti-Grb2 Antibody (M331)

SKU: GM3311

Product Details

Bulk Order Anti-Grb2 Antibody

Related Products

Recently Viewed