Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Antibody (1D4)

Name: Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Antibody
Brand: Biosensis
SKU: M-1376-250
Price: 337.0 USD
Availability: InStock

Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) mouse monoclonal primary antibody detects bovine, chicken, human, mouse, pig, and rat Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), and is purified. It is validated for use in ICC, IHC-Frozen, WB.

Human neuroblastoma SH-SY5Y cells stained with Mouse monoclonal antibody to Glyceraldehyde 3-Phosphate Dehydrogenase [1D4] M-1376-250 (green), chicken antibody to neurofilament H C-1386-50 (red) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.

Blots of mice peripheral nerves blotted with M-1376-250 for use as a western blotting control. Note the single sharp band corresponding to GAPDH.

Left: Staining of GAPDH in HeLa cells with mouse anti-GAPDH antibody (1:100, green) by Immunocytochemistry. Blue: DAPI nuclear stain. The GAPDH antibody produces strong cytoplasmic staining of healthy cells. Right: Western blot analysis of cell lysates probed with mouse antibody to GAPDH (1:2,000). [1] protein standard, [2] HEK293, [3] HeLa, [4] SH-SY5Y, [5] COS1, [6] NIH-3T3, and [7] C6. The GAPDH antibody reveals a single band at about 37 kDa in all cell lines. GAPDH is a "house keeping‚Äù protein, the level of which is relatively unaffected by most experimental manipulations, and, as a result, this antibody has been widely used as a western blotting loading control.

Human neuroblastoma SH-SY5Y cells stained with Mouse monoclonal antibody to Glyceraldehyde 3-Phosphate Dehydrogenase [1D4] M-1376-250 (green), chicken antibody to neurofilament H C-1386-50 (red) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.

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Species Reactivity

Bovine, Chicken, Human, Mouse, Pig, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1376-250

Volume/Size: 250 µg

Variant

Price:

$337.00

Quantity:

Ships: 5-7 business days

Product Details

Target

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Scientific Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.

Format

Purified

Clonality

Monoclonal

Clone

1D4

Isotype

IgM

Applications

ICC, IHC, WB

Host Species

Mouse

Molecular Weight

36 kDa

Immunogen

Purified pig GAPDH

Immunogen Species

Pig

Species Reactivity

Bovine, Chicken, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 250 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein L purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB. Human, rat, mouse, bovine, Porcine, Chicken.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

38 kDa BFA-dependent ADP-ribosylation substrate; Glyceraldehyde-3-phosphate dehydrogenase; aging associated gene 9 protein; GAPDH; Aging-associated gene 9 protein; GAPD; BARS-38; G3PDH; cb609; GPDH; EC 1.2.1.12; Epididymis secretory sperm binding protein Li 162eP; G3P_HUMAN; G3PD; G3PDH; GAPD; GAPDH; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS; p38 component; OCT1 coactivator in S phase; 38-KD component; peptidyl cysteine S nitrosylase GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; wu:fb33a10

UniProt ID

P00355
UniProt Summary: Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.

NCBI Gene ID

396823

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Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Antibody (1D4)

SKU: M-1376-250

Product Details

Bulk Order Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) Antibody

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