Anti-Glutathione peroxidase 1 (GPx-1) Antibody

Name: Anti-Glutathione peroxidase 1 (GPx-1) Antibody
Brand: Biosensis
SKU: R-1588-100
Price: 337.0 USD
Availability: InStock

Our Anti-Glutathione peroxidase 1 (GPx-1) rabbit polyclonal primary antibody detects human and rat Glutathione peroxidase 1 (GPx-1), and is whole serum. It is validated for use in ELISA, IHC-Paraffin-embedded, WB.

Immunohistochemical staining of neurons in rat brain tissue. Incubated with Rabbit polyclonal antibody to glutathione peroxidase 1 (R-1588-100) at 1:100. Secondary antibody donkey anti-rabbit conjugated to Cy3 at 1:100.

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Species Reactivity

Human, Rat

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1588-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Product Details

Target

Glutathione peroxidase 1 (GPx-1)

Scientific Background

Glutathione peroxidase 1 has a role in detoxification of hydrogen peroxide and is one of the most important antioxidant enzymes in humans. It exists as a homotetramer which localises to the cytoplasm. It belongs to the glutathione peroxidase family. Glutathione peroxidase 1 is one of few proteins in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. This protein has a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine repeats. The allele with five alanine repeats is significantly associated with breast cancer risk. At least two alternatively spliced isoforms have been identified.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ELISA, IHC, WB

Host Species

Rabbit

Molecular Weight

22 kDa (see application details)

Immunogen

A synthetic peptide (RRYSRRFQTIDIEPDIEALL) corresponding to the amino acids 175-194 of human glutathione peroxidase 1 ( GPx-1) conjugated to diphtheria toxin has been used as the immunogen.

Immunogen Species

Human

Species Reactivity

Human, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized serum with 0.02% thimerosal

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:200-1:1000

Application Details

This antibody works in IHC on frozen or wax embedded tissues. Antigen retrieval has been used in testing but may not be necessary. This antiserum has extremely high titer, it stains human and rat glial cells intensely and to some extent also stains neurons. recognizes a 22 kD protein in human brain tissue and Sigma glutathione peroxidase (G-4013) isolated from human erythrocytes.

Conjugate

Unconjugated

Specificity

Human and rat cellular selenium dependent glutathione peroxidase. Human; rat;.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Glutathione peroxidase 1; GPx-1; GSHPx-1; Cellular glutathione peroxidase; GPX1;

UniProt ID

P07203

Shipping

25°C (ambient)

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Anti-Glutathione peroxidase 1 (GPx-1) Antibody

SKU: R-1588-100

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