Our Anti-GluR2/3 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat GluR2/3 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR2/3 protein.
The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990;Hollmann and Heinemann, 1994). The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory (Duprat et al., 2003;Seidenman et al., 2003;Chung et al., 2003;Yan et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
GRIA2/3
100 kDa
Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GluR2/3, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Chicken, Human, Mouse, Zebrafish
AB_2492130
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~100 kDa GluR2/3 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Chung HJ, Steinberg JP, Huganir RL, Linden DJ (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755. PMID: 12805550
Duprat F, Daw M, Lim W, Collingridge G, Isaac J (2003) GluR2 protein-protein interactions and the regulation of AMPA receptors during synaptic plasticity. Philos Trans R Soc Lond B Biol Sci 358:715-720. PMID: 12740117
Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108. PMID: 8210177
Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560. PMID: 2166337
