Anti-GluR2/3 Antibody

Name: Anti-GluR2/3 Antibody
Brand: PhosphoSolutions
SKU: 905-GluR2/3
Price: 456.0 USD
Availability: InStock

Our Anti-GluR2/3 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects rat GluR2/3 and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of rat hippocampal lysate showing specific immunolabeling of the ~100 kDa GluR2/3 protein.

Click on image to zoom

Species Reactivity

Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 905-GluR2/3

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluR2/3

Scientific Background

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990;Hollmann and Heinemann, 1994). The GluR2 subunit is widely expressed throughout the nervous system where it is thought to play key roles in synaptic plasticity and learning and memory (Duprat et al., 2003;Seidenman et al., 2003;Chung et al., 2003;Yan et al., 2002).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

GRIA2/3

Molecular Weight

100 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat GluR2/3, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Rat

Expected Reactivity

Chicken, Human, Mouse, Zebrafish

Antibody Registry ID

AB_2492130

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from rabbit serum by affinity purification using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~100 kDa GluR2/3 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

AMPA 2; AMPA selective glutamate receptor 2; AMPA-selective glutamate receptor 2; AMPA2; GluA2; GLUR 2; GLUR B; GluR K2; GluR-2; GluR-B; GluR-K2; GLUR2; GLURB; Glutamate receptor 2; Glutamate receptor ionotropic AMPA 2; Glutamate receptor ionotropic; Gria2; GRIA2_HUMAN; HBGR2; AMPA selective glutamate receptor 3; GluR K3; GLURC; GLUTAMATE RECEPTOR 3; Glutamate receptor ionotropic AMPA 3; GRIA3

UniProt ID

P19491
UniProt Summary: Ionotropic glutamate receptor that functions as a ligand-gated cation channel, gated by L-glutamate and glutamatergic agonists such as alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA), quisqualic acid, and kainic acid (PubMed:20614889, PubMed:31300657, PubMed:8003671). L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system and plays an important role in fast excitatory synaptic transmission (PubMed:14687553). Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse upon entry of monovalent and divalent cations such as sodium and calcium (PubMed:20614889, PubMed:8003671). The receptor then desensitizes rapidly and enters in a transient inactive state, characterized by the presence of bound agonist (By similarity). In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of L-glutamate (By similarity). Through complex formation with NSG1, GRIP1 and STX12 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity). {ECO:0000250|UniProtKB:P19491, ECO:0000269|PubMed:14687553, ECO:0000269|PubMed:20614889, ECO:0000269|PubMed:31300657, ECO:0000269|PubMed:8003671}.

NCBI Gene ID

29627

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Chung HJ, Steinberg JP, Huganir RL, Linden DJ (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300:1751-1755. PMID: 12805550
Duprat F, Daw M, Lim W, Collingridge G, Isaac J (2003) GluR2 protein-protein interactions and the regulation of AMPA receptors during synaptic plasticity. Philos Trans R Soc Lond B Biol Sci 358:715-720. PMID: 12740117
Hollmann M, Heinemann S (1994) Cloned glutamate receptors. Annu Rev Neurosci 17:31-108. PMID: 8210177
Keinänen K, Wisden W, Sommer B, Werner P, Herb A, Verdoorn TA, Sakmann B, Seeburg PH (1990) A family of AMPA-selective glutamate receptors. Science 249:556-560. PMID: 2166337
Seidenman KJ, Steinberg JP, Huganir R, Malinow R (2003) Glutamate receptor subunit 2 serine 880 phosphorylation modulates synaptic transmission and mediates plasticity in CA1 pyramidal cells. J Neurosci 23:9220-9228. PMID: 14534256
Yan, J., Zhang, Y., Jia, Z., Taverna, F.A., McDonald, R.J., Muller, R.U. and Roder, J.C., 2002. Place-cell impairment in glutamate receptor 2 mutant mice. Journal of Neuroscience, 22(3), pp.RC204-RC204. PMID: 11826150

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Anti-GluR2/3 Antibody

SKU: 905-GluR2/3

Product Details

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