Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb (N59/20)

Name: Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-097
Price: 470.0 USD
Availability: InStock

Our chicken Anti-GluN2B/NR2B glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from clone N59/20. It detects human, mouse, and rat GluN2B/NR2B glutamate receptor and is purified by affinity chromatography. It works in WB, IHC, ICC, IP, ELISA.

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus showing neurons in CA4 region stained by anti-GluN2B recombinant chicken monoclonal antibody (Cat. 78-097) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

$Western blotting of rat brain membrane fraction with anti-GluN2B/NR2B glutamate receptor mouse mAb (Cat. 75-097) and Aves Labs anti-GluN2B/NR2B recombinant chicken mAb (Cat. 78-097) antibodies. The mouse mAb and chicken mAb were detected with anti-mouse-HRP and anti-chicken-HRP (Cat. 80-1000-HRP), respectively. Aves Labs Recombinant Chicken anti-GluN2B/NR2B mAb recognizes endogenous NR2B with similar sensitivity as the anti-GluN2B/NR2B mouse mAb in the$

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex stained by anti-GluN2B recombinant chicken monoclonal antibody using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei. Anti-GluN2B specifically stains the neurons in layers II/III of the cerebral cortex.

Representative section of formalin fixed, paraffin-embedded rat brain hippocampus showing neurons in CA4 region stained by anti-GluN2B recombinant chicken monoclonal antibody (Cat. 78-097) using goat anti-chicken IgY CF®488 secondary antibody (Cat. 80-1001-488) (visualized in green). The stained sections were mounted with Antibodies Incorporated Fluoroshield with DAPI and DABCO mounting medium (Cat. AR-6505). DAPI nuclear stain (blue) shows cell nuclei.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Go to Full Product Details

Data Sheet

KO Validated

Trial Size

SKU: 78-097

Volume: 100 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-5 business days

Product Details

Target

GluN2B/NR2B glutamate receptor

Scientific Background

GluN2B/NR2b glutamate receptor, also known as Glutamate/N-methyl D-aspartate/NMDA receptor subtype 2B, is a member of the NMDA receptor family. NMDA receptors are expressed throughout the CNS in postsynaptic cell membranes. GluN2B is a major NMDAR subtype found in cortical and hippocampal regions of the adult brain. NMDA receptors are involved with memory and learning. Mutations in the gene encoding GluN2B are associated with autism, LennoxGastaut and West Syndromes.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

N59/20

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

Grin2b

Molecular Weight

166 kDa

Immunogen

Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (accession number Q00960) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N59/2 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N59/2 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:500
IHC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with GluN2A/NR2A

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Glutamate receptor ionotropic, NMDA 2B (GluN2B) (Glutamate [NMDA] receptor subunit epsilon-2) (N-methyl D-aspartate receptor subtype 2B) (NMDAR2B) (NR2B)

UniProt ID

Q00960

Shipping

Shipped on ice packs

Recover password

Create my account

Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb (N59/20)

SKU: 78-097

Product Details

Bulk Order Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb

Related Products

Recently Viewed