Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb (BSA and Azide free) (N59/36)

Name: Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb (BSA and Azide free)
Brand: Aves Labs
SKU: 78-101-CF
Price: 522.0 USD
Availability: InStock

Our chicken Anti-GluN2B/NR2B glutamate receptor carrier-free recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N59/36. It detects human, mouse, and rat GluN2B/NR2B glutamate receptor and is purified by affinity chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb (BSA and Azide free)

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

Unconjugated

490

550

594

650

Biotin

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Data Sheet

Trial Size

SKU: 78-101-CF

Volume: 100 µL

Variant

Price:

$522.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluN2B/NR2B glutamate receptor

Scientific Background

GluN2B/NR2B glutamate receptor, also known as Glutamate/N-methyl D-aspartate/NMDA receptor subtype 2B, is a member of the NMDA receptor family. NMDA receptors are expressed throughout the CNS in postsynaptic cell membranes. GluN2B is a major NMDAR subtype found in cortical and hippocampal regions of the adult brain. NMDA receptors are involved with memory and learning. Mutations in the gene encoding GluN2B are associated with autism, LennoxGastaut and West Syndromes.

Format

Purified by affinity chromatography

Concentration

Lot dependent: provided at 0.6-1.0 mg/mL

Clonality

Recombinant

Clone

N59/36

Isotype

IgY

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

Grin2b

Molecular Weight

166 kDa

Excitation / Emission

Unconjugated

Immunogen

Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (accession number Q00960) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N59/36 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N59/36 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

Buffer

PBS

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000
IHC: 1:1000
ICC: 1:500

Conjugate

Unconjugated, Carrier Free

Specificity

Does not cross-react with GluN2A/NR2A

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

AW490526; Glutamate receptor ionotropic; EIEE27; GluN2B; Glutamate [NMDA receptor subunit epsilon 2; Glutamate [NMDA receptor subunit epsilon-2; Glutamate [NMDA receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; Glutamate Receptor Ionotropic N Methyl D Aspartate 2B; NMDAR2B; Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B; NR2B; Glutamate receptor ionotropic NMDA2B; Glutamate receptor subunit epsilon 2; Glutamate receptor ionotropic NMDA2B (epsilon 2; GRIN 2B; GRIN2B; hNR 3; hNR3; MGC142178; MGC142180; MRD6; N methyl D asparate receptor channel subunit epsilon 2; N methyl D aspartate receptor subtype 2B; N methyl D aspartate receptor subunit 2B; N methyl D aspartate receptor subunit 3; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NMDA NR2B; NMDA R2B; NMDAR2B; NMDE2; NMDE2_HUMAN; NME2; NR2B; NR3; NMDA 2B

UniProt ID

Q00960
UniProt Summary: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+). Participates in synaptic plasticity for learning and memory formation by contributing to the long-term depression (LTD) of hippocampus membrane currents. Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium. Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.

NCBI Gene ID

24410

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Anti-GluN2B/NR2B Glutamate Receptor Recombinant Chicken Chimeric mAb (BSA and Azide free) (N59/36)

SKU: 78-101-CF

Product Details

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