Anti-GFP Antibody (N86/38)

Name: Anti-GFP Antibody
Brand: NeuroMab
SKU: 75-132
Price: 374.0 USD
Availability: InStock

Our Anti-GFP mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N86/38. It detects GFP, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Transfected cell immunoblot extracts of COS cells transfected with GFP; GFP-tagged HCN2, FGF11/FHF3 FGF13/FHF2 and BKBeta2; or untagged Kv2.1 plasmid and probed with N86/38 TC supe.

Transfected cell immunofluorescence: COS cells expressing GFP-FHF2A. Blue = Hoechst nuclear stain, Green = GFP, Red = N86/38.

Transfected cell immunoblot extracts of COS cells transfected with GFP-tagged MHC, CFP-tagged MHC, YFP-tagged Histone H3.3 or untagged Kv2.1 plasmid and probed with N86/38 (left) or N52A/42 (right) TC supe.

MDA-MB-231-β1-GFP cells were stained for (A) EEA1, (B) LAMP1, (C) GM130, (D) TGN46, (E) calnexin (all magenta), and DNA (DAPI, blue). GFP is shown in green. Image from publication CC-BY-4.0. PMID: 35752364

Transfected cell immunoblot extracts of COS cells transfected with GFP; GFP-tagged HCN2, FGF11/FHF3 FGF13/FHF2 and BKBeta2; or untagged Kv2.1 plasmid and probed with N86/38 TC supe.

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

Go to Full Product Details

Data Sheet

SKU: 75-132

Volume: 100 µL

Variant

Price:

$374

Quantity:

Product Details

Target

GFP

Target Description

Green fluorescent protein (GFP) from the jellyfish Aequorea Victoria is widely used as a marker of protein expression or targeting in cells or model organisms.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N86/38

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

GFP

Molecular Weight

27 kDa

Antigen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

Antibody Registry ID

AB_10672529

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.58

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Green fluorescent protein

UniProt Details

UniProt (Human): N/A
UniProt (Immunogen Species): P42212

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Anti-GFP Antibody (N86/38)

SKU: 75-132

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