Anti-GFAP Recombinant Guinea Pig Chimeric mAb (N206A/8)
Our guinea pig Anti-GFAP recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N206A/8. It detects drosophila, guinea pig, human, mouse, polar bear, and rat GFAP and is purified by affinity chromatography. KO validated. Works in ELISA, ICC, IHC, WB.
![Immunohistochemistry for detection of GFAP in adult rat cerebellum using Anti-GFAP Recombinant Chimeric Guinea Pig Monoclonal antibody [N206A/8] (Cat No. 74-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-GFAP antibody (Cat No. 74-240) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain cerebellum.](http://www.antibodiesinc.com/cdn/shop/files/74-240-ihc-1_120x120.jpg?v=1778860430)
![Western blot detection of GFAP using Anti-GFAP Recombinant Chimeric Guinea Pig Monoclonal antibody [N206A/8] (Cat No. 74-240). 10-20% Tris-Glycine Gel was loaded with rat brain membrane fraction. Proteins were transferred to PVDF membrane and blocked with 5% non-fat milk powder. Anti-GFAP antibody was incubated with sample at a concentration of 1 ug/ml and then detected via chemiluminescence (10 min) using a Goat Anti-Guinea Pig H+L, HRP conjugated secondary antibody. Anti-GFAP recognizes endogenous GFAP in the rat brain membrane fraction at the expected molecular weight of ~50 kDa. ](http://www.antibodiesinc.com/cdn/shop/files/74-240-wb-1_120x120.jpg?v=1778860430)
![Immunohistochemistry for detection of GFAP in adult rat hippocampus using Anti-GFAP Recombinant Chimeric Guinea Pig Monoclonal antibody [N206A/8] (Cat No. 74-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-GFAP antibody (Cat No. 74-240) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain hippocampus.](http://www.antibodiesinc.com/cdn/shop/files/74-240-ihc-2_120x120.jpg?v=1778860430)
![Immunohistochemistry for detection of GFAP in adult rat hippocampus using Anti-GFAP Recombinant Chimeric Guinea Pig Monoclonal antibody [N206A/8] (Cat No. 74-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-GFAP antibody (Cat No. 74-240) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain hippocampus. ](http://www.antibodiesinc.com/cdn/shop/files/74-240-ihc-3_120x120.jpg?v=1778860430)
![Immunohistochemistry for detection of GFAP in adult rat cerebellum using Anti-GFAP Recombinant Chimeric Guinea Pig Monoclonal antibody [N206A/8] (Cat No. 74-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-GFAP antibody (Cat No. 74-240) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain cerebellum.](http://www.antibodiesinc.com/cdn/shop/files/74-240-ihc-1_1600x.jpg?v=1778860430)
Immunohistochemistry for detection of GFAP in adult rat cerebellum using Anti-GFAP Recombinant Chimeric Guinea Pig Monoclonal antibody [N206A/8] (Cat No. 74-240). Tissue was fixed with 4% paraformaldehyde and paraffin embedded. Heat induced antigen retrieval was performed using citrate buffer (Cat No. AR-6544) and then blocked with Immuno Protein Blocking Solution with Goat Serum (Cat No. AR-6591). The anti-GFAP antibody (Cat No. 74-240) was incubated with the sample at a dilution of 1:250 at 4C overnight. After washing with PBST, the sections were stained using goat anti-guinea pig 488 secondary antibody (green). DAPI nuclear stain (blue) shows cell nuclei. Anti-GFAP specifically stains the astrocytes in the rat brain cerebellum.
KO Validated
Trial Size