Anti-GDNF family receptor alpha-3 (GFR alpha-3) Antibody

Name: Anti-GDNF family receptor alpha-3 (GFR alpha-3) Antibody
Brand: Biosensis
SKU: C-1661-100
Price: 457.0 USD
Availability: InStock

Our Anti-GDNF family receptor alpha-3 (GFR alpha-3) chicken polyclonal primary antibody detects human GDNF family receptor alpha-3 (GFR alpha-3), and is affinity-purified. It is validated for use in ICC, WB.

Western-Blot detection of human GFRα-3, using Chicken polyclonal antibody to human GFRα-3. GFRα-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Line 1. Prestained protein ladder (Naxo 8003). Line 2. hGFRα-1. Primary antibody dilution 1:5000 was used.

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Species Reactivity

Human

Applications

ICC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1661-100

Volume/Size: 100 µg

Variant

Price:

$457.00

Quantity:

Product Details

Target

GDNF family receptor alpha-3 (GFR alpha-3)

Scientific Background

The GFRa-3 is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor and a member of the GDNF receptor family. It forms a signaling receptor complex with RET tyrosine kinase receptor and binds the ligand, artemin.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, WB

Host Species

Chicken

Immunogen

Recombinant His-tagged human GFRa-3 protein produced using CHO-based cell line. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.

Immunogen Species

Human

Species Reactivity

Human

Storage

Store antibody stock solution at +4°C upon receipt. DO NOT FREEZE. This product is an (NH4)2SO4 precipitate. To use, mix well by pipetting or vortexing gently prior use, then withdraw the desired amount of antibody stock solution and dilute into your antibody dilution buffer and use normally.
Alternatively, material can be centrifuged at 10K x g for 5 minutes and the liquid sulfate solution carefully removed. Then precipitated antibody can be resuspended in 0.1M P, 250 mM NaCL, pH 7.4 solution. The solution should then be divided into working aliquots and stored at -80°C until used. Prevent multiple freeze thaw cycles of aliquots for best product life. Store at 2-8°C upon receipt; DO NOT FREEZE. As product is (NH4)2SO4 (ammonium sulfate) precipitate, mix well by pipetting or vortexing prior to use. See reconstitution instructions for more information.

Physical State

Liquid

Buffer

Concentrated ammonium sulphate in PBS pH 7.4 containing no preservatives.

Dilution Ranges

WB: 1:5000-1:10000
ICC: 1:1500-1:3000

Conjugate

Unconjugated

Specificity

Human GFRa-3. No cross-reactivity with hGFRa-1, hGFRa-2, hGFRa-4.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

GDNF receptor alpha-3; GDNFR-alpha-3; GFR-alpha-3; GFRA3;

UniProt ID

O60609

Shipping

2-8°C (on cold packs)

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Anti-GDNF family receptor alpha-3 (GFR alpha-3) Antibody

SKU: C-1661-100

Product Details

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