Anti-GAPDH Antibody

Name: Anti-GAPDH Antibody
Brand: Biosensis
SKU: R-1701-100
Price: 327.0 USD
Availability: InStock

Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), and is whole serum. It is validated for use in ICC, WB.

HeLa cell extract blotted with rabbit anti-GAPDH. Note the single sharp band corresponding to GAPDH.

HeLa cells stained with rabbit anti-GAPDH (red), monoclonal to TAF15 (green) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.

$Left: HeLa cells stained with rabbit antibody to GAPDH (red, 1:2,000) by Immunocytochemistry. Blue: Hoechst nuclear stain. The GAPDH antibody produces diffuse cytoplasmic staining. Right: Western blot analysis of cell cytosolic- or nuclear-enriched fractions, using rabbit antibody to GAPDH (red, 1:20,000, lanes 2-5). [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. A strong band at 37 kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse antibody to the nuclear RNA binding protein SF3B4 (M-1576-100, green, 1:1,000). In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.$

HeLa cell extract blotted with rabbit anti-GAPDH. Note the single sharp band corresponding to GAPDH.

Click on image to zoom

Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1701-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Scientific Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.

Format

Whole serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Molecular Weight

~36-38 kDa

Immunogen

Full length recombinant human GAPDH expressed in and purified from E. coli

Immunogen Species

Human

Species Reactivity

Human, Mouse

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized with sodium azide.

Dilution Ranges

WB: 1:10000-1:30000
ICC: 1:1000-1:10000

Conjugate

Unconjugated

Specificity

Specific for the ~36-38 kDa Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; GAPD; G3PDH; GPDH

UniProt ID

P04406

Shipping

25°C (ambient)

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Anti-GAPDH Antibody

SKU: R-1701-100

Product Details

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