Anti-GAPDH Antibody

Name: Anti-GAPDH Antibody
Brand: Biosensis
SKU: R-1701-100
Price: 337.0 USD
Availability: InStock

Our Anti-Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) rabbit polyclonal primary antibody detects human, mouse, other mammals and (predicted) Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH), and is whole serum. It is validated for use in ICC, WB.

HeLa cell extract blotted with rabbit anti-GAPDH. Note the single sharp band corresponding to GAPDH.

HeLa cells stained with rabbit anti-GAPDH (red), monoclonal to TAF15 (green) and DNA (blue). The antibody reveals strong cytoplasmic staining for GAPDH.

$Left: HeLa cells stained with rabbit antibody to GAPDH (red, 1:2,000) by Immunocytochemistry. Blue: Hoechst nuclear stain. The GAPDH antibody produces diffuse cytoplasmic staining. Right: Western blot analysis of cell cytosolic- or nuclear-enriched fractions, using rabbit antibody to GAPDH (red, 1:20,000, lanes 2-5). [1] protein standard, [2] NIH-3T3 cytosolic, [3] NIH-3T3 nuclear, [4] HeLa cytosolic, and [5] HeLa nuclear fractions. A strong band at 37 kDa corresponds to GAPDH protein, mainly detected in the cytosolic fractions. The same blot was simultaneously probed with mouse antibody to the nuclear RNA binding protein SF3B4 (M-1576-100, green, 1:1,000). In contrast to GAPDH, SF3B4 is exclusively expressed in the nuclear fraction.$

HeLa cell extract blotted with rabbit anti-GAPDH. Note the single sharp band corresponding to GAPDH.

Click on image to zoom

Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1701-100

Volume/Size: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH)

Scientific Background

Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) is a metabolic enzyme responsible for catalyzing one step in the glycolytic pathway, the reversible oxidative phosphorylation of glyceraldehyde 3-phosphate. GAPDH may have other roles in the activation of transcription and in the regulation of apoptosis as well as Alzheimer's disease and Huntington's disease. The immunogen used to raise this particular antibody was extensively purified pig GAPDH. This antibody can be used as a loading control for western blotting experiments, allowing comparison between the level of this protein and others in a cell or tissue.

Format

Whole serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Molecular Weight

~36-38 kDa

Immunogen

Full length recombinant human GAPDH expressed in and purified from E. coli

Immunogen Species

Human

Species Reactivity

Human, Mouse

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized with sodium azide.

Dilution Ranges

WB: 1:10000-1:30000
ICC: 1:1000-1:10000

Conjugate

Unconjugated

Specificity

Specific for the ~36-38 kDa Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) protein.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

38 kDa BFA-dependent ADP-ribosylation substrate; Glyceraldehyde-3-phosphate dehydrogenase; aging associated gene 9 protein; GAPDH; Aging-associated gene 9 protein; GAPD; BARS-38; G3PDH; cb609; GPDH; EC 1.2.1.12; Epididymis secretory sperm binding protein Li 162eP; G3P_HUMAN; G3PD; G3PDH; GAPD; GAPDH; Glyceraldehyde 3 phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; HEL-S-162eP; KNC-NDS6; MGC102544; MGC102546; MGC103190; MGC103191; MGC105239; MGC127711; MGC88685; OCAS; p38 component; OCT1 coactivator in S phase; 38-KD component; peptidyl cysteine S nitrosylase GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; wu:fb33a10

UniProt ID

P04406
UniProt Summary: Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis. Also shows nitrosylase activity, thereby playing a role in nuclear functions. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation. Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.

NCBI Gene ID

2597

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Anti-GAPDH Antibody

SKU: R-1701-100

Product Details

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