Our Anti-Gap-43 (Ser41) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Gap-43 (Ser41) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific immunolabeling of the ~50 kDa Gap-43 protein phosphorylated at Ser41 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Gap-43 is thought to have an important role in development and plasticity because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration (Benowitz and Routtenberg, 1997). There is also evidence from knockout animals that Gap-43 serves to amplify pathfinding signals from the growth cone (Strittmatter et al., 1995). Gap-43 is thought to mediate at least some of these effects via interaction with actin. Importantly, phosphorylation at Ser-41 by protein kinase C (Catalog No. 1609-PKC) modulates the interaction of Gap-43 with actin (He et al., 1997) and may also affect neurotransmitter release during forms of plasticity like LTP (Hulo et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
GAP43
50 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser41of rat GAP43, conjugated to keyhole limpet hemocyanin (KLH).
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~50 kDa Gap-43 protein phosphorylated at Ser41. In some tissues the antibody also recognizes a higher molecular weight protein that is also recognized by the pan Gap-43 antibody, which may be a Gap-43 aggregate or oligomer. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser41
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
Axonal membrane protein GAP 43 antibody, Axonal membrane protein GAP-43 antibody, B 50 antibody, Calmodulin binding protein P 57 antibody, F1 antibody, GAP 43 antibody, GAP-43 antibody, GAP43 antibody, Growth Associated Protein 43 antibody, Growth-associated protein 43 antibody, Nerve Growth Related Peptide antibody, Nerve growth related peptide GAP43 antibody, NEUM_HUMAN antibody, Neural phosphoprotein B 50 antibody, Neural phosphoprotein B-50 antibody, Neuromodulin antibody, Neuron growth associated protein 43 antibody, PP46 antibody, Protein F1 antibody, QtrA-11580 antibody, QtrA-13071 antibody
Gopalakrishna, R., et al. 2008. A direct redox regulation of protein kinase C isoenzymes mediates oxidant-induced neuritogenesis in PC12 cells. Journal of Biological Chemistry, 283(21), pp.14430-14444.
