Our Anti-GABAB Receptor (Ser923), R1-Subunit rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAB Receptor (Ser923), R1-Subunit and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of rat synaptic membrane lysate showing specific immunolabeling of the ~102 kDa GABAB R1 protein phosphorylated at Ser923 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABA-A and the GABA-B subtype of receptors. GABA-B receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. Phosphorylation enhances GABA-B receptor effector coupling (Couve et al., 2004). Phosphorylation of Ser-923 is thought to be important in the regulation of GABA-B receptor function.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
GABBR1
102 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser923 of the R1-subunit of rat GABAB receptor, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Bovine, Human, Non-Human Primate
AB_2492118
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~102 kDa GABAB R1 phosphorylated at Ser923. Immunolabeling is completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser923
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
