Our Anti-GABAA Receptor γ2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor γ2 and is neat pooled serum. It is great for use in WB, IHC.
Western blot of 10 µg of rat hippocampal lysate showing specific immunolabeling of the ~46 kDa γ2-subunit of the GABAA-R.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, co-expression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of β-subunits of the receptor has been shown to modulate GABA-A-R function (Brandon et al., 2003).
Neat Pooled Serum
Polyclonal
IgG
IHC, WB
Rabbit
GABRG2
46 kDa
Synthetic peptide corresponding to amino acid residues specific to the γ2 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Chicken, Human, Non-Human Primate, Zebrafish
AB_2492114
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Pooled neat serum
neat serum
WB: 1:1000
WB Brain: 1:1000
IHC: 1:400
Unconjugated
Specific for endogenous levels of the ~46 kDa γ2-subunit of the GABAA receptor.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Fagan, M.P., et al. 2020. . Essential and sex-specific effects of mGluR5 in ventromedial hypothalamus regulating estrogen signaling and glucose balance. Proceedings of the National Academy of Sciences.
