Anti-GABAA Receptor ß3 Antibody

Name: Anti-GABAA Receptor ß3 Antibody
Brand: PhosphoSolutions
SKU: 863A-GB3C
Price: 456.0 USD
Availability: InStock

Our Anti-GABAA Receptor ß3 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor ß3 and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.

Western blot of rat brain lysate showing specific immunolabeling of the ~53 kDa β3-subunit of the GABAA-R.

Immunofluorescence of mouse retina showing staining of GABAA-R, β3-subunit (Cat. : 863A-GB3C, green, 1:300) and calbindin (red). The blue is DAPI staining nuclear DNA. Photo courtesy of Dr. Arlene Hirano, UCLA.

Western blot of rat cortical neurons showing specific immunolabeling of the ~53 kDa β3-subunit of the GABAA-R (1:1000). Image kindly provided by Lidong Liu, University of British Columbia, Vancouver.

$Immunoblots showing GABAA Receptor α2 (Cat. 822-GA2CL) and GABAA Receptor ß3 (Cat. 863A-GB3C) subunit expression in the cytosolic (S2), extrasynaptic (non-PSD), and synaptic (PSD) fractions of untreated, bicuculline-pretreated (20μM, 1h), or netrin-1 treated (250ng/ml, 1h) rat hippocampal neuronal cultures.Image from publication CC-BY-4.0. PMID: 36323250$

Western blot of rat brain lysate showing specific immunolabeling of the ~53 kDa β3-subunit of the GABAA-R.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: 863A-GB3C

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABAA Receptor ß3

Scientific Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α- subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Host Species

Rabbit

Gene Name

GABRB3

Molecular Weight

53 kDa

Immunogen

Fusion protein from the cytoplasmic loop of the beta 3 subunit of rat GABAA receptor.

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2650497

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IHC: 1:300

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~53 kDa β3-subunit of the GABAA receptor.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

ECA5; GABA alpha receptor beta-2 subunit; GABA A receptor beta 3; GABA(A receptor subunit beta-3; GABAA receptor beta 3 subunit; GABAA receptor subunit beta 3; GABR B3; Gabrb3; Gamma aminobutyric acid (GABA A receptor beta 3; Gamma aminobutyric acid receptor subunit beta 3; Gamma-aminobutyric acid receptor subunit beta-3; GBRB3_HUMAN; MGC9051; GABA(A) receptor subunit beta-3; Gamma aminobutyric acid

UniProt ID

P63079
UniProt Summary: Beta subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed:14993607, PubMed:18514161, PubMed:22243422, PubMed:22303015, PubMed:24909990, PubMed:26950270, PubMed:30602789). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed:24909990, PubMed:30140029, PubMed:30602789). GABAARs containing beta-3/GABRB3 subunit are found at both synaptic and extrasynaptic sites (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed:14993607, PubMed:22303015, PubMed:26950270, PubMed:30602789). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (PubMed:22303015, PubMed:26950270). GABAARs containing alpha-1 and beta-3 subunits exhibit synaptogenic activity; the gamma-2 subunit being necessary but not sufficient to induce rapid synaptic contacts formation (PubMed:25489750). Extrasynaptic beta-3 receptors contribute to the tonic GABAergic inhibition (By similarity). GABAARs containing alpha-1, beta-3 and epsilon subunits may also permit spontaneous chloride channel activity while preserving the structural information required for GABA-gated openings (By similarity). Beta-containing GABAARs can simultaneously bind GABA and histamine where histamine binds at the interface of two neighboring beta subunits, which may be involved in the regulation of sleep and wakefulness (PubMed:18281286, PubMed:24909990, PubMed:35355020). Plays an important role in somatosensation and in the production of antinociception (By similarity). {ECO:0000250|UniProtKB:P63079, ECO:0000250|UniProtKB:P63080, ECO:0000269|PubMed:14993607, ECO:0000269|PubMed:18281286, ECO:0000269|PubMed:18514161, ECO:0000269|PubMed:22243422, ECO:0000269|PubMed:22303015, ECO:0000269|PubMed:24909990, ECO:0000269|PubMed:25489750, ECO:0000269|PubMed:26950270, ECO:0000269|PubMed:30140029, ECO:0000269|PubMed:30602789, ECO:0000269|PubMed:35355020}.

NCBI Gene ID

24922

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Blue Ice

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Anti-GABAA Receptor ß3 Antibody

SKU: 863A-GB3C

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