Anti-GABAA Receptor α5 Antibody
Our Anti-GABAA Receptor α5 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat GABAA Receptor α5 and is antigen affinity purified pooled serum. It is great for use in WB, IHC.
SKU: 846A-GA5C
Product Details
GABAA Receptor α5
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).
Antigen Affinity Purified Pooled Serum
Polyclonal
IgG
IHC, WB
Rabbit
GABRA5
55 kDa
Fusion protein from the cytoplasmic loop of the α5 subunit of rat GABAA receptor.
Mouse, Rat
Bovine, Canine, Chicken, Human, Non-Human Primate, Zebrafish
AB_2797145
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
IHC: Not listed
WB Brain: 1:1000
IHC: Not listed
Unconjugated
Specific for endogenous levels of the ~55 kDa α5-subunit of the GABAA receptor.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
GAα5 antibody, GAA5 antibody, GABA(A) receptor subunit alpha-5 antibody, GABRα5 antibody, Gabra5 antibody, Gamma aminobutyric acid GABA A receptor alphα5 antibody, Gamma aminobutyric acid GABA A receptor alphα5 precursor antibody, Gamma aminobutyric acid receptor alphα5 subunit precursor GABA A receptor antibody, Gamma-aminobutyric acid receptor subunit alpha-5 antibody, GBRA5_HUMAN antibody, GC138184 antibody
UniProt (Human): P31644
UniProt (Immunogen Species): P19969
UniProt (Immunogen Species): P19969
29707
Product Specific Protocols
- Western Blot Protocol: Download