Anti-c-FOS Antibody (N486/32)

1 Citation

Our Anti-c-FOS mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N486/32. It detects human, mouse, and rat c-FOS, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunohistochemistry against hippocampus from adult rats treated with pilocarpine to induce seizures (top) and with saline (bottom).
Immunoblot against crude brain low-speed pellets from untreated adult rats (RBLSP), adult rats treated with kainic acid to induce seizures (RBLSP seiz) and adult rats treated with saline (RBLSP non-seiz) probed with N486/32 (left), N408/62 (middle) and N52A/42 (right) TC supe.
Rat spinal cord sections labeling cFOS (green, Cat 75-506) to confirm excitation of aerents by DREAD. Two “experimental” rats (A,B) received excitatory DREADDS injections into DRGs (AAV2-hSyn-hM3Dq-mCherry). Three weeks later rats were injected with CNO (4 mg/kg), rested 30 minutes followed by running one hour on traeadmill, then sacrificed. Two control rats, (C) had DRGs injected with mCherry sham (AAV2-hSyn-mCherry), or (D) no DRG injections. Image from publication CC-BY-4.0. PMID: 36090254
Immunohistochemistry against hippocampus from adult rats treated with pilocarpine to induce seizures (top) and with saline (bottom).
Roll over image to zoom in Click on image to zoom
Immunohistochemistry against hippocampus from adult rats treated with pilocarpine to induce seizures (top) and with saline (bottom).
Immunoblot against crude brain low-speed pellets from untreated adult rats (RBLSP), adult rats treated with kainic acid to induce seizures (RBLSP seiz) and adult rats treated with saline (RBLSP non-seiz) probed with N486/32 (left), N408/62 (middle) and N52A/42 (right) TC supe.
Rat spinal cord sections labeling cFOS (green, Cat 75-506) to confirm excitation of aerents by DREAD. Two “experimental” rats (A,B) received excitatory DREADDS injections into DRGs (AAV2-hSyn-hM3Dq-mCherry). Three weeks later rats were injected with CNO (4 mg/kg), rested 30 minutes followed by running one hour on traeadmill, then sacrificed. Two control rats, (C) had DRGs injected with mCherry sham (AAV2-hSyn-mCherry), or (D) no DRG injections. Image from publication CC-BY-4.0. PMID: 36090254

Human, Mouse, Rat
ICC, IHC, WB
Mouse
490
550
594
650
Carrier-Free

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Data Sheet

SKU: 75-506

Volume: 100 µL
Price:
$329.00
Ships: 1-2 business days

Product Specific References for Applications and Species

  • Immunohistochemistry: Rat
Immunohistochemistry: Rat
PMID Dilution Publication
36090254not listedEisdorfer, J.T., et al. 2022. Chemogenetic modulation of sensory afferents induces locomotor changes and plasticity after spinal cord injury. Frontiers in Molecular Neuroscience, 872634.

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