Anti-FLASH (C-terminus) Antibody

Name: Anti-FLASH (C-terminus) Antibody
Brand: PhosphoSolutions
SKU: 24168
Price: 456.0 USD
Availability: InStock

Our FLASH rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human FLASH and is antigen affinity purified. It is great for use in WB.

Western Blot of NIH3T3 cell lysate showing specific immunolableing of FLASH.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: 24168

Title: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

FLASH

Scientific Background

Fas is a cell-surface receptor molecule that relays apoptotic (cell death) signals into cells. When Fas is activated by binding of its ligand, the proteolytic protein caspase-8 is recruited to a signalling complex known as DISC by binding to a Fas-associated adapter protein. A new protein, FLASH, contains a motif with oligomerizing activity whose sequence is similar to that of the Caenorhabditis elegans protein CED-4, and another domain (DRD domain) that interacts with a death-effector domain in caspase-8 or in the adapter protein. FLASH is likely a component of the DISC signalling complex and is necessary for the activation of caspase-8 in Fas-mediated apoptosis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

CASP8AP2

Immunogen

Synthetic peptide corresponding to amino acids from the C-terminal region of human FLASH protein

Immunogen Species

Human

Species Reactivity

Human

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.02% NaN3

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000

Specificity

Reacts with the C-terminal region of human FLASH protein

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CED-4; CASP8-associated protein 2; RIP25; FLASH homolog RIP25; FLICE associated huge; FLICE-associated huge protein; human FLASH; CASP8AP; CED4

UniProt ID

Q9UKL3
UniProt Summary: Participates in TNF-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF-kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Involved for histone gene transcription and progression through S phase: required for the generation of mature histone mRNAs following the 3' end cleavage of histone pre-mRNAs.

NCBI Gene ID

9994

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Anti-FLASH (C-terminus) Antibody

SKU: 24168

Product Details

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