Our Anti-FANCI (Ser559) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human FANCI (Ser559) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of Hela cell lysate treated with UV (~254nm) for 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al., 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation of Ser-556 and Ser-559 are highly conserved and have been determined to be ATM/ATR kinase substrates for the FA-DNA pathway (Smogorzewska et al., 2007). Furthermore, the phosphorylation of Ser-556 and Ser-559 have been shown to play key roles in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014). Research continues to determine if one site plays more of a role in the DNA repair/damage checkpoint of the FA pathway and stabilization of the ID2 complex.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
FANCI
150 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser559 of human FANCI, conjugated to keyhole limpet hemocyanin (KLH).
Human
Non-Human Primate
AB_2636808
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:250
Unconjugated
Specific for endogenous levels of the ~150 kDa FANCI protein phosphorylated at Ser559. Immunolabeling is increased when HeLa cells are treated with 256nm UV light for 20 minutes and completely eliminated by treatment with λ-phosphatase.
Phosphorylated
Ser559
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
FANCI antibody, FANCI gene antibody, FANCI_HUMAN antibody, Fanconi anemia group I protein antibody, Fanconi anemia complementation group I antibody, FLJ10719 antibody, FLJ14658 antibody, KIAA1794 antibody, Protein FACI antibody, Protein FANCI antibody,
Cheung, R., et al. 2017. Ubiquitination-linked phosphorylation of the FANCI S/TQ cluster contributes to activation of the Fanconi anemia I/D2 complex. Cell Reports, 19(12), pp.2432-2440.
