Anti-FANCI (Ser559) Antibody

Name: Anti-FANCI (Ser559) Antibody
Brand: PhosphoSolutions
SKU: p171-559
Price: 456.0 USD
Availability: InStock

Our Anti-FANCI (Ser559) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human FANCI (Ser559) and is antigen affinity purified from pooled serum. It is great for use in WB.

Western blot of Hela cell lysate treated with UV (~254nm) for 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Western blot of HeLa cell lysates that had been treated with UV (~254 nm) for 0’ or 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559.

Western blot of Hela cell lysate treated with UV (~254nm) for 20’ showing specific immunolabeling of the ~150 kDa FANCI protein phosphorylated at Ser559 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

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Species Reactivity

Human

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p171-559

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

FANCI (Ser559)

Scientific Background

Fanconi anemia, FA, is a rare disorder where cells cannot prevent, repair, or tolerate DNA damage, leading to cancer, progressive bone marrow failure and developmental abnormalities (Ishiai et al., 2008). 16 genes have been implicated in FA, and their products constitute a common FA pathway (Walden and Deans 2014). FANCI (Fanconi anemia complementation group I), is one of two substrates for monoubiquitnation by the FANCL-containing core complex, and is crucial for DNA repair via FAP and intrastrand cross-links (Walden and Deans, 2014). Phosphorylation of Ser-556 and Ser-559 are highly conserved and have been determined to be ATM/ATR kinase substrates for the FA-DNA pathway (Smogorzewska et al., 2007). Furthermore, the phosphorylation of Ser-556 and Ser-559 have been shown to play key roles in FANCI physically associating with FANCD2, stabilizing the ID2 complex, and supporting its DNA binding and subsequent D2-ubiquitination (Walden and Deans, 2014). Research continues to determine if one site plays more of a role in the DNA repair/damage checkpoint of the FA pathway and stabilization of the ID2 complex.

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

FANCI

Molecular Weight

150 kDa

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser559 of human FANCI, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Human

Species Reactivity

Human

Expected Reactivity

Non-Human Primate

Antibody Registry ID

AB_2636808

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:250

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~150 kDa FANCI protein phosphorylated at Ser559. Immunolabeling is increased when HeLa cells are treated with 256nm UV light for 20 minutes and completely eliminated by treatment with λ-phosphatase.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser559

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

FANCI; FANCI gene; FANCI_HUMAN; Fanconi anemia group I protein; Fanconi anemia complementation group I; FLJ10719; FLJ14658; KIAA1794; Protein FACI; Protein FANCI ,

UniProt ID

Q9NVI1
UniProt Summary: Plays an essential role in the repair of DNA double-strand breaks by homologous recombination and in the repair of interstrand DNA cross-links (ICLs) by promoting FANCD2 monoubiquitination by FANCL and participating in recruitment to DNA repair sites (PubMed:17412408, PubMed:17460694, PubMed:17452773, PubMed:19111657, PubMed:36385258). The FANCI-FANCD2 complex binds and scans double-stranded DNA (dsDNA) for DNA damage; this complex stalls at DNA junctions between double-stranded DNA and single-stranded DNA (PubMed:19589784). Participates in S phase and G2 phase checkpoint activation upon DNA damage (PubMed:25862789). {ECO:0000250|UniProtKB:B0I564, ECO:0000269|PubMed:17412408, ECO:0000269|PubMed:17452773, ECO:0000269|PubMed:17460694, ECO:0000269|PubMed:19111657, ECO:0000269|PubMed:19589784, ECO:0000269|PubMed:25862789, ECO:0000269|PubMed:36385258}.

NCBI Gene ID

55215

Shipping

Blue Ice

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Anti-FANCI (Ser559) Antibody

SKU: p171-559

Product Details

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