Our Anti-EWS primary antibody from PhosphoSolutions is mouse monoclonal. It detects canine, human, mouse, and rat EWS and is Protein G purified. It is great for use in WB, IHC, ICC.
Western blot of HeLa cell lysate showing specific immunolabeling of the ~85 kDa EWS protein.
The Ewing Sarcoma Breakpoint Region 1 (EWSR1) gene encodes the EWS protein, which along with FUS and TAF15 comprises the FET family of RNA-binding proteins. EWS is primarily expressed in the nucleus, but has also been found in the cytoplasm of secretory cell types (Andersson et al., 2008). Defects of EWSR1 are known to cause Ewing sarcoma and have been implicated in amyotrophic lateral sclerosis (ALS) (Svetoni et al., 2016). Recently, EWS has been shown to directly interact with oncogenic ETS transcription factors in the promotion of prostate cancer (Kedage et al., 2016).
Protein G Purified
Monoclonal
5H7
IgG2b
ICC, IHC, WB
Mouse
EWSR1
85 kDa
Full length recombinant human EWS expressed in and purified from E. coli.
Canine, Human, Mouse, Rat
AB_2650521
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Protein G purified from culture supernatant.
PBS + 50% glycerol and 5 mm NaN3
WB: 1:1000
IHC: 1:1000
ICC: 1:1000
Unconjugated
Specific for endogenous levels of the ~85 kDa EWS protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Andersson, M.K., Ståhlberg, A., Arvidsson, Y., Olofsson, A., Semb, H., Stenman, G., Nilsson, O. and Åman, P., 2008. The multifunctional FUS, EWS and TAF15 proto-oncoproteins show cell type-specific expression patterns and involvement in cell spreading and stress response. BMC Cell Biology, 9(1), p.37. PMID: 18620564
Svetoni, F., Frisone, P. and Paronetto, M.P., 2016. Role of FET proteins in neurodegenerative disorders. RNA Biology, 13(11), pp.1089-1102. PMID: 27415968
Kedage, V., Selvaraj, N., Nicholas, T.R., Budka, J.A., Plotnik, J.P., Jerde, T.J. and Hollenhorst, P.C., 2016. An interaction with Ewing’s sarcoma breakpoint protein EWS defines a specific oncogenic mechanism of ETS factors rearranged in prostate cancer. Cell Reports, 17(5), pp.1289-1301. PMID: 27783944
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