Anti-Endoribonuclease Dicer Antibody

Name: Anti-Endoribonuclease Dicer Antibody
Brand: Biosensis
SKU: C-1557-100
Price: 347.0 USD
Availability: InStock

Our Anti-Endoribonuclease Dicer chicken polyclonal primary antibody detects mouse and rat Endoribonuclease Dicer, and is affinity-purified. It is validated for use in ICC, IHC-Frozen, WB.

$Western blotting with Chicken polyclonal antibody to mouse Dicer protein (C-1557-100) in mouse brain extracts. Figure A: Mouse brain cytosolic extract. Detection at 0.03 µg/mL. Protein loaded 20 µg, 10 µg and 5 µg. Figure B: Mouse brain subcellular fractionation. Detection at 0.03 µg/mL. Protein loaded 10 µg.$

Western blotting with Chicken polyclonal antibody to mouse Dicer protein (C-1557-100) in mouse brain extracts. Figure A: Mouse brain cytosolic extract. Detection at 0.03 µg/mL. Protein loaded 20 µg, 10 µg and 5 µg. Figure B: Mouse brain subcellular fractionation. Detection at 0.03 µg/mL. Protein loaded 10 µg.

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Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1557-100

Volume/Size: 100 µL

Variant

Price:

$347.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Endoribonuclease Dicer

Scientific Background

Dicer is the rate limiting enzyme in the formation of mature microRNAs.

Format

Affinity-purified

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Molecular Weight

218 kDa

Immunogen

A synthetic peptide from mouse Dicer protein (1388-1405 aa) conjugated to KLH.

Immunogen Species

Mouse

Species Reactivity

Mouse, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. At least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability and at 2-8°C with an appropriate antibacterial agent. Avoid freeze-thaw cycles.

Physical State

Liquid

Buffer

PBS with 0.02% sodium azide.

Dilution Ranges

WB: 1:1000-1:3000
IHC: 1:200-1:500

Application Details

The recommended concentration for IHC in formalin fixed and paraffin embedded tissues and formalin/acetone fixed tissues is 1:200-1:500. For WB, the recommended concentration is 1:1000 to 1:3000.

Conjugate

Unconjugated

Specificity

Specificity confirmed by IHC and WB in mouse brain. mouse; rat;.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

DCR1; Endoribonuclease Dicer; Dicer1e; Double-strand-specific ribonuclease mDCR-1; HERNA; Dicer1; K12H4.8-LIKE; Mdcr; MNG1; RMSE2; Dcr-1 homolog; dicer 1; ribonuclease type III; helicase MOI; helicase with RNAse motif

UniProt ID

Q8R418
UniProt Summary: Double-stranded RNA (dsRNA) endoribonuclease playing a central role in short dsRNA-mediated post-transcriptional gene silencing. Cleaves naturally occurring long dsRNAs and short hairpin pre-microRNAs (miRNA) into fragments of twenty-one to twenty-three nucleotides with 3' overhang of two nucleotides, producing respectively short interfering RNAs (siRNA) and mature microRNAs. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. Gene silencing mediated by siRNAs, also called RNA interference, controls the elimination of transcripts from mobile and repetitive DNA elements of the genome but also the degradation of exogenous RNA of viral origin for instance. The miRNA pathway on the other side is a mean to specifically regulate the expression of target genes.

NCBI Gene ID

23405

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25°C (ambient)

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Anti-Endoribonuclease Dicer Antibody

SKU: C-1557-100

Product Details

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