Our Anti-Elk-1 (Ser383) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects mouse and rat Elk-1 (Ser383) and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of recombinant Elk-1 showing specific immunolabeling of the ~46 kDa Elk-1 phosphorylated at Ser383 in the right lane (-). Phosphospecificity is shown in the left lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors (Hill and Treisman, 1995). Elk-1 is thought to be phosphorylated by MAP kinase at Ser-383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1 (Li et al., 2003). Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus (Thiels et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
ELK1
46 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 of human Elk-1, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
Canine, Human, Non-Human Primate, Zebrafish
AB_2492085
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for ~46 kDa Elk-1 phosphorylated at Ser383. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide.
Phosphorylated
Ser383
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Hill, C.S. and Treisman, R., 1995. Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell, 80(2), pp.199-211. PMID: 8521730
Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D. and Martins‐Green, M., 2003. MAP kinase phosphorylation‐dependent activation of Elk‐1 leads to activation of the co‐activator p300. The EMBO Journal, 22(2), pp.281-291. PMID: 8521730
Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., 2002. Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1. Journal of Neuroscience, 22(6), pp.2054-2062. PMID: 8521730
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