Our Anti-eIF4A2 rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse eIF4A2 and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of mouse testes lysate showing specific immunolabeling of the ~46 kDa eukaryotic initiation factor 4A2, eIF4A2, protein.
The eIF4A, eukaryotic initiation factor 4A, are a set of proteins, eIF4A1, eIF4A2, and eIF4A3, that belong to the extensive DEAD-box RNA helicase family (Weinstein et al., 1997). They are involved in many aspects of RNA metabolism by virtue of their RNA-binding capacity and ATPase activity (Lu et al., 2014). eIF4A2 is a highly conserved gene for one of the protein-synthesis initiation factors involved in the binding of mRNA to the ribosome (Gingras et al., 1999). In humans eIF4A2 is expressed in all normal tissues, though highly expressed in skeletal muscle (Sudo et al., 1995). Studies suggest eIF4A2 may play a role in some cancers, specifically breast and lung cancer, along with melanoma (Shaoyan et al., 2013).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, WB
Rabbit
EIF4A2
46 kDa
Synthetic peptide from the N-terminal region of the human eIF4A2 protein.
Human
Human, Mouse
Bovine, Non-Human Primate, Rat
AB_2630324
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via chromatography on peptide immunogen column.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~46 kDa eIF4A2 protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Q14240
UniProt Summary: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Lu, W.T., Wilczynska, A., Smith, E. and Bushell, M., 2014. The diverse roles of the eIF4A family: you are the company you keep. Biochemical Society transactions , 42(1): 166-172.PMID: 24450646
Shaoyan, X., Juanjuan, Y., Yalan, T., Ping, H., Jianzhong, L. and Qinian, W., 2013. Downregulation of EIF4A2 in non–small-cell lung cancer associates with poor prognosis. Clinical Lung Cancer, 14(6), pp.658-665. PMID: 23867391
Gingras, A.C., Raught, B. and Sonenberg, N., 1999. eIF4 initiation factors: effectors of mRNA recruitment to ribosomes and regulators of translation. Annual Review of Biochemistry, 68(1), pp.913-963. PMID: 10872469
Weinstein, D.C., Honoré, E. and Hemmati-Brivanlou, A., 1997. Epidermal induction and inhibition of neural fate by translation initiation factor 4AIII. Development, 124(21), pp.4235-4242. PMID: 9334272
Sudo, K., Takahashi, E. and Nakamura, Y., 1995. Isolation and mapping of the human EIF4A2 gene homologous to the murine protein synthesis initiation factor 4A-II gene Eif4a2. Cytogenetic and Genome Research, 71(4), pp.385-388. PMID: 8521730
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