Anti-EB3 (Ser-162), Phosphospecific Antibody

Name: Anti-EB3 (Ser-162), Phosphospecific Antibody
Brand: PhosphoSolutions
SKU: EP4981
Price: 348.0 USD
Availability: InStock

Our EB3 (Ser-162) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat EB3 (Ser-162) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (EM5091) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, WB

Host Species

Rabbit

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Data Sheet

SKU: EP4981

Volume: 100 µL

Variant

Price:

$348.00

Quantity:

Ships: 1-2 business days

Product Details

Target

EB3 (Ser-162)

Scientific Background

Microtubles (MTs) are oriented with a fast growing plus-end and a slower growing minus-end. The MT plus-end is a crucial site for the regulation of MT dynamics and MT association with different cellular organelles by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions. The End-Binding (EB) family of +TIPs includes EB1 (MAPRE1), EB2 (MAPRE2, RP1), and EB3 (MAPRE3, EBF3). EB proteins are ubiquitiously expressed +TIPs that can dimerize through a coiled-coil C-terminal region, and bind MTs through an N-terminal calponin homology domain. EB proteins can stabilize MTs, increase MT dynamics, and suppress MT pauses. Site specific phosphorylation may regulate EB activity. EB3 Ser-162 phosphorylation destabilizes EB3 dimer and reduces MT growth, while aurora-kinase induced Ser-176 phosphorylation regulates EB3 protein stability during mitosis.

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, WB

Host Species

Rabbit

Gene Name

MAPRE3

Molecular Weight

Immunogen

Phospho-EB3 (Ser-162) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 162 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3, and is not conserved in EB1 and EB2.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 32 kDa* protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser-162

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

EB3; Microtubule RP/EB; EBF3-S; RP3; APC binding protein; EB1 protein family member 3; end-binding protein 3; MAPRE3; APC; End-binding. RP3

UniProt ID

Q9UPY8
UniProt Summary: Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules.

NCBI Gene ID

22924

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Anti-EB3 (Ser-162), Phosphospecific Antibody

SKU: EP4981

Product Details

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