Anti-EB3 Antibody (KT53)

Name: Anti-EB3 Antibody
Brand: PhosphoSolutions
SKU: EM5101
Price: 292.0 USD
Availability: InStock

Our EB3 primary antibody from PhosphoSolutions is rat monoclonal. It detects human, mouse, and rat EB3 and is Protein G purified. It is great for use in WB, ICC.

Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals EM5041 anti-EB1 (lane 1), EM5061 anti-EB1/2/3 (lane 2), EM5081 anti-EB2 (lane 3), EM5101 anti-EB3 (lane 4), and EM5091 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific:HRP (RS3121).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, WB

Host Species

Rat

Go to Full Product Details

Data Sheet

SKU: EM5101

Volume: 100 µL

Variant

Price:

$292.00

Quantity:

Ships: 1-2 business days

Product Details

Target

EB3

Scientific Background

The microtubule (MT) plus-end is a crucial site for the regulation of MT dynamics and interactions by several groups of plus-end tracking proteins (+TIPs). These +TIPs form comet-like accumulations at the plus ends of MTs to regulate MT dynamics and interactions with organelles and macromolecular complexes. The +TIPs include diverse groups of proteins, such as motor and nonmotor proteins, MT polymerases and depolymerases as well as various regulatory and adaptor proteins. The CLIP-associated protein (CLASP) family includes CLASP1 and CLASP2 proteins, which are expressed as long (α) and short (β) isoforms. Thse +TIPs conatin an N-terminal TOG domain, multiple TOG-like domains, and a basic and serine-rich motif (SxIP). The TOG domain facilitates interaction with tubulin dimers, while the SxIP motif promotes interaciton with EB1 and MTs. A C-terminal domain is involved in interaction with CLIPs, as well as several other proteins. CLASPs are MT stabilizing fators that localize to mitotic spindles, kinetochores, and the midbody. CLASPs are important for cell division, and may regulate cell migration and neuronal growth cone motility.

Format

Protein G Purified

Clonality

Monoclonal

Clone

KT53

Isotype

IgG2a

Applications

ELISA, ICC, WB

Host Species

Rat

Gene Name

MAPRE3

Molecular Weight

Immunogen

Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Buffer

PBS + 0.05% NaN3

Dilution Ranges

WB: 1:1000
ICC: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 32 kDa* prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

EB3; Microtubule RP/EB; EBF3-S; RP3; APC binding protein; EB1 protein family member 3; end-binding protein 3; MAPRE3; APC; End-binding. RP3

UniProt ID

Q9UPY8
UniProt Summary: Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth. May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes. Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement. Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules.

NCBI Gene ID

22924

Shipping

Blue Ice

Recover password

Create my account

Anti-EB3 Antibody (KT53)

SKU: EM5101

Product Details

Bulk Order Anti-EB3 Antibody

Related Products

Recently Viewed