Our Anti-Dynamin rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Dynamin and is antigen affinity purified from pooled serum. It is great for use in WB.
Western Blot of rat cortical lysate showing labeling of the ~95 kDa dynamin protein.
Dynamin is a member of a group of nerve terminal proteins called dephosphins that regulate synaptic vesicle endocytosis (Cousin et al., 2001; Graham et al., 2002; Tsuboi et al., 2002). There are 3 known isoforms of Dynamin, each having several splice variants as well. Dynamin I is expressed only in neurons whereas Dynamin II is ubiquitously expressed and Dynamin III is found primarily in the testes. Dynamin 1 is phosphorylated by PKC and dephosphorylated by calcineurin.
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
DNM1
95 kDa
Synthetic peptide corresponding to amino acid residues from the N-terminal region of human dynamin, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Rat
Bovine, Chicken, Non-Human Primate
AB_2492081
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from rabbit serum by affinity purification via chromatography on an affinity column made with the N-terminal peptide used as antigen.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~95 kDa dynamin protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Cousin, M.A., Tan, T.C. and Robinson, P.J., 2001. Protein phosphorylation is required for endocytosis in nerve terminals: potential role for the dephosphins dynamin I and synaptojanin, but not AP180 or amphiphysin. Journal of Neurochemistry, 76(1), pp.105-116. PMID: 11145983
Graham, M.E., O'Callaghan, D.W., McMahon, H.T. and Burgoyne, R.D., 2002. Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size. Proceedings of the National Academy of Sciences, 99(10), pp.7124-7129. PMID: 11997474
Tsuboi, T., Terakawa, S., Scalettar, B.A., Fantus, C., Roder, J. and Jeromin, A., 2002. Sweeping model of dynamin activity - Visualization of coupling between exocytosis and endocytosis under an evanescent wave microscope with green fluorescent proteins. Journal of Biological Chemistry, 277(18), pp.15957-15961. PMID: 11856729
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