Anti-Dopamine beta-hydroxylase (DBH) Antibody

Name: Anti-Dopamine beta-hydroxylase (DBH) Antibody
Brand: Biosensis
SKU: R-108-100
Price: 327.0 USD
Availability: InStock

Our Anti-Dopamine beta-hydroxylase (DBH) rabbit polyclonal primary antibody detects bovine, guinea pig, human, and rat Dopamine beta-hydroxylase (DBH), and is whole serum. It is validated for use in IHC-Frozen, Neutralization, WB.

Immunohistochemical staining of Dopamine beta-Hydroxylase (DBH) in guinea pig brain stem using rabbit polyclonal to native bovine DBH, catalogue number R-108-100 .

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Species Reactivity

Bovine, Guinea Pig, Human, Pig, Rat

Applications

IHC, Neutralize, WB

Host Species

Rabbit

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Data Sheet

SKU: R-108-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Dopamine beta-hydroxylase (DBH)

Scientific Background

DBH is an oxireductase belonging to the copper type II ascorbate-dependent monooxygenase family. DBH exists as a homotetramer composed of two non-covalently bound disulfide-linked dimers. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It binds 2 copper ions and 1 PQQ per subunit . Depending on the presence of a signal peptide, DBH can exist in both soluble and membrane-bound forms.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC, Neutralize, WB

Host Species

Rabbit

Immunogen

Native enzyme prepared from Bovine adrenal glands. Purity was greater than 95% by PAGE.

Immunogen Species

Bovine

Species Reactivity

Bovine, Guinea Pig, Human, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution keep aliquots at -20°C for a higher stability, and at 2-8°C with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.

Physical State

Lyophilized

Dilution Ranges

WB: 1:500-1:2000
IHC: 1:500-1:2000

Application Details

Radioimmunoassay and inhibition of enzymatic activity: Recommended to be used at a dilution of 1:500 to 1:2000.

Conjugate

Unconjugated

Specificity

This antiserum has been extensively characterised in several laboratories. It reacts with a single band on Western blot of bovine adrenal homogenates and stains only cells known to contain DBH, such as sympathetic neurons, adrenal medullary cells and central adrenergic neurons. Staining is abolished by preincubation with the enzyme. This antibody is known to react with bovine, human, guinea pig and rat DBH.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Dopamine beta-hydroxylase; Dopamine beta-monooxygenase; Soluble dopamine beta-hydroxylase;

UniProt ID

P15101

Shipping

25°C (ambient)

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Anti-Dopamine beta-hydroxylase (DBH) Antibody

SKU: R-108-100

Product Details

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