Our Anti-Dopamine ß-Hydroxylase, C-Terminus sheep polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and non-human primate Dopamine ß-Hydroxylase, C-Terminus and is antigen affinity purified from pooled serum. It is great for use in WB.
Western blot of human adrenal medulla lysate showing specific immunolabeling of the ~75 kDa DBH protein.
DBH catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. DBH antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson’s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). The expression of DBH is also elevated during stress (Sabban and Kvetnansky, 2001).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Sheep
DBH
75 kDa
Synthetic peptide from the C-terminal region of human dopamine β-hydroxylase (DBH), conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse, Non-Human Primate
AB_2492074
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled sheep serum by affinity purification using a column matrix to which the peptide immunogen was coupled.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~75 kDa DBH protein.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Kish, S.J., Kalasinsky, K.S., Derkach, P., Schmunk, G.A., Guttman, M., Ang, L., Adams, V., Furukawa, Y. and Haycock, J.W., 2001. Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology, 24(5), p.561. PMID: 11282256
Sabban, E.L. and Kvetňanský, R., 2001. Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events. Trends in Neurosciences, 24(2), pp.91-98. PMID: 11164939
Zhu, M.Y., Klimek, V., Dilley, G.E., Haycock, J.W., Stockmeier, C., Overholser, J.C., Meltzer, H.Y. and Ordway, G.A., 1999. Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biological Psychiatry, 46(9), pp.1275-1286. PMID: 10560033
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