Anti-DNMT3L Antibody (N117/9)

Name: Anti-DNMT3L Antibody
Brand: NeuroMab
SKU: 75-153
Price: 329.0 USD
Availability: InStock

Our Anti-DNMT3L mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N117/9. It detects human DNMT3L, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Transfected cell immunofluorescence: COS cells expressing Myc-tagged human DNMT3L. Red = rabbit anti-Myc, Green = N117/9, Blue = Hoechst nuclear stain.

Transfected cell immunoblot: extracts of COS cells transiently transfected with untagged Kv2.1 and Flag-tagged DNMT3L, DNMT3A and DNMT3B plasmids and probed with N117/9 TC supe (left) and mouse anti-Flag (right).

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Species Reactivity

Human

Applications

ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-153

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

DNMT3L

Scientific Background

DNA (cytosine-5)-methyltransferase 3-like or DNMT3L is encoded by the gene DNMT3L. DNMT3l is a nuclear, catalytically inactive, regulatory factor of DNA methyltransferases. DNMT3L mediates transcriptional repression through interaction with histone deacetylase 1. It is thought to be required for the establishment of maternal genomic imprints. DNMT3L is expressed in several tissues including testis, ovary, and thymus. Diseases associated with DNMT3L include Testicular Spermatocytic Seminoma and Immunodeficiency-Centromeric Instability-Facial Anomalies Syndrome.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N117/9

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

DNMT3L

Molecular Weight

45 kDa

Antigen

Fusion protein amino acids 1-387 (full-length) of human DNMT3L (accession number BAA95556) produced recombinantly in E. Coli

Species Reactivity

Human

Antibody Registry ID

AB_2094287

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.79

Conjugate

Unconjugated

Specificity

No cross-reactivity against DNMT3A or DNMT3B

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

DNA (cytosine-5)-methyltransferase 3-like

UniProt ID

BAA95556

Product Specific References for Applications and Species

Publications: Unspecified

Publications: Unspecified
PMID	Publication
25421653	Satterlee, J.S., et al. 2015. Community resources and technologies developed through the NIH Roadmap Epigenomics Program.. Methods Molecular Biology, 27-49.

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Anti-DNMT3L Antibody (N117/9)

SKU: 75-153

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-DNMT3L Antibody

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