Anti-DGCR8 (Ser377) Antibody
Our Anti-DGCR8 (Ser377) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human and mouse DGCR8 (Ser377) and is antigen affinity purified from pooled serum. It is great for use in WB.
SKU: p1035-377
Product Details
DGCR8 (Ser377)
The Drosha-DGCR8 microprocessor complex is required for microRNA (miRNA) biogenesis. DGCR8 (DiGeorge Syndrome Critical Region 8) recognizes the RNA substrate, whereas Drosha functions as the endonuclease. DGCR8, which contains two double-stranded RNA (dsRNA)-binding domains, interacts with the pri-miRNA and functions as the molecular anchor that measures the distance from the ds-RNA-ssRNA junction and directs Drosha cleavage 11bp away (Han, J., et al, 2006). The efficiency of Drosha cleavage increases in the presence of heme and promotes the formation of highly ordered DGCR8 structures upon binding to RNA (Faller, M., et al, 2010).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
WB
Rabbit
DGCR8
120 kDa
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser377 of human DGCR8, conjugated to keyhole limpet hemocyanin (KLH).
Human, Mouse
AB_2492071
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Liquid
Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phospho peptide affinity columns.
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
WB: 1:1000
Unconjugated
Specific for endogenous levels of the ~120 kDa DGCR8 protein phosphorylated at Ser377. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.
Phosphorylated
Ser377
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
DGCRK6 antibody, C22orf12 antibody, D16H22S788E antibody, D16Wis2 antibody, DGCR 8 antibody, Dgcr8 antibody, DGCR8 microprocessor complex subunit antibody, DGCR8_HUMAN antibody, DGCRK 6 antibody, DiGeorge syndrome critical region 8 antibody, DiGeorge syndrome critical region gene 8 antibody, Gy1 antibody, Microprocessor complex subunit DGCR8 antibody, pasha antibody
UniProt (Human): Q8WYQ5
UniProt (Immunogen Species): Q8WYQ5
UniProt (Immunogen Species): Q8WYQ5
1644
Product Specific Protocols
- Western Blot Protocol: Download
General References
- Han, J., Lee, Y., Yeom, K.H., Nam, J.W., Heo, I., Rhee, J.K., Sohn, S.Y., Cho, Y., Zhang, B.T. and Kim, V.N., 2006. Molecular basis for the recognition of primary microRNAs by the Drosha-DGCR8 complex. Cell, 125(5), pp.887-901. PMID: 16751099
- Faller, M., Toso, D., Matsunaga, M., Atanasov, I., Senturia, R., Chen, Y., Zhou, Z.H. and Guo, F., 2010. DGCR8 recognizes primary transcripts of microRNAs through highly cooperative binding and formation of higher-order structures. RNA, 16(8), pp.1570-1583. PMID: 20558544